Separation of overlapped particles in digital holographic microscopy
Ahmed El Mallahi,Frank Dubois +1 more
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TLDR
A procedure to separate aggregates of overlapped particles in digital holograms, based on a focus plane analysis applied to each particle, is presented, successfully demonstrated experimentally on different types of aggregates.Abstract:
In this paper, we present a procedure to separate aggregates of overlapped particles in digital holograms, based on a focus plane analysis applied to each particle. The method can be applied either on phase or on amplitude objects, according that each object has a border in one focus plane. Numerical simulations are performed to quantify the robustness of the process by increasing the overlapping areas between the particles. The separation algorithm is successfully demonstrated experimentally on different types of aggregates.read more
Citations
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Holographic deconvolution microscopy for high resolution particle tracking
TL;DR: In this article, the authors used Rayleigh-Sommerfeld backpropagation to reconstruct the three-dimensional light field responsible for the recorded intensity in an in-line hologram.
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Refocus criterion for both phase and amplitude objects in digital holographic microscopy
TL;DR: For digital holographic microscopy applications, the focus criterion is modified based on the integration of the amplitude modulus to make possible its use regardless of the phase or amplitude nature of the objects under test.
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High throughput holographic imaging-in-flow for the analysis of a wide plankton size range
TL;DR: A Digital Holographic Microscope working with a partial coherent source specifically adapted to perform high throughput recording of holograms of plankton organisms in-flow, in a size range of 3 µm-300 µm, which is of importance for this kind of applications.
New Techniques in Digital Holography
Pascal Picart,Michael Atlan,Marc Brunel,Sébastien Coëtmellec,Jean-Michel Desse,Frank Dubois,Ahmed El Mallahi,Marc Georges,Michel Gross,Denis Lebrun,Pierre Marquet,Minetti Christophe,Nicolas Pavillon,Benjamin Rappaz,Nicolas Verrier +14 more
TL;DR: In this paper, a state-of-the-art state of the art of important advances in the field of digital holography is presented. But the authors are interested in detailing advances related to the fundamentals of holographic metrology, digital holographic microscopy, and full-field vibrometry.
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Refinement of particle detection by the hybrid method in digital in-line holography
TL;DR: A refinement procedure is developed that distinguishes such erroneous particles from accurately detected ones and further separates individual particles in digital in-line holography and confirms the usefulness of the proposed method.
References
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Survey over image thresholding techniques and quantitative performance evaluation
Mehmet Sezgin,Bulent Sankur +1 more
TL;DR: 40 selected thresholding methods from various categories are compared in the context of nondestructive testing applications as well as for document images, and the thresholding algorithms that perform uniformly better over nonde- structive testing and document image applications are identified.
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Generalizing the hough transform to detect arbitrary shapes
TL;DR: It is shown how the boundaries of an arbitrary non-analytic shape can be used to construct a mapping between image space and Hough transform space, which makes the generalized Houghtransform a kind of universal transform which can beused to find arbitrarily complex shapes.
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Digital recording and numerical reconstruction of holograms
Ulf Schnars,Werner Jüptner +1 more
TL;DR: The principles and major applications of digital recording and numerical reconstruction of holograms (digital holography) are described, which are applied to measure shape and surface deformation of opaque bodies and refractive index fields within transparent media.
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Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy.
Pierre Marquet,Benjamin Rappaz,Pierre J. Magistretti,Etienne Cuche,Yves Emery,Tristan Colomb,Christian Depeursinge +6 more
TL;DR: A digital holographic microscope, in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells, is developed, based on an original numerical algorithm presented in detail elsewhere.