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Sero-epidemiological survey for alveolar echinococcosis (by Em2-ELISA) of blood donors in an endemic area of Switzerland

Bruno Gottstein, +7 more
- 01 Nov 1987 - 
- Vol. 81, Iss: 6, pp 960-964
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TLDR
Sera from 17,166 blood donors living in 10 cantons of northern Switzerland in an area endemic for Echinococcus multilocularis were investigated by serological survey for alveolar echinococcosis, finding 2 asymptomatic clinical cases of AE within this population of blood donors.
Abstract: 
Sera from 17 166 blood donors living in 10 cantons of northern Switzerland in an area endemic for Echinococcus multilocularis were investigated by serological survey for alveolar echinococcosis (AE). A highly species-specific antigen (Em2) and a commonly used E. granulosus hydatid fluid antigen (EgHF) were compared for their suitability in seroepidemiology. EgHF showed a degree of nonspecificity which did not allow direct detection of AE cases. Antibody reaction with Em2 resulted in the detection of 2 asymptomatic clinical cases of AE (seroprevalence 0·01%) within this population of blood donors. A further 4 persons were positive in Em2-ELISA. These 4 persons had negative imaging studies and will be followed serologically and clinically.

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Citations
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Journal ArticleDOI

Improved primary immunodiagnosis of alveolar echinococcosis in humans by an enzyme-linked immunosorbent assay using the Em2plus antigen.

Bruno Gottstein, +3 more
- 01 Feb 1993 - 
Abstract: Alveolar echinococcosis (AE) in humans is generally a fatal disease when not diagnosed early enough to provide curative treatment such as radical surgery. Immunodiagnosis for early detection of AE was improved by the isolation of an affinity-purified metacestode Em2 antigen and by the synthesis of recombinant Echinococcus multilocularis antigen II/3-10. Both antigens were individually assessed by enzyme-linked immunosorbent assay (ELISA) and demonstrated high specificities and diagnostic sensitivities, although both missed approximately 4 to 11% of diagnostic cases of AE. To provide an optimal serodiagnostic test, we investigated the two purified antigens by using a test employing a mixture of both purified antigens (designated Em2plus antigen) in one assay. For comparative purposes, crude E. multilocularis and Echinococcus granulosus metacestode antigens were investigated as well. The Em2plus ELISA proved to be the optimal diagnostic test with the highest diagnostic sensitivity, 97%, in serum samples from 140 patients with AE and an overall specificity of 99% for infections due to other Echinococcus and non-Echinococcus parasites. The new test combination (Em2plus ELISA) is suggested for the serodiagnosis of AE in patients and for seroepidemiological surveys.

Improved Primary Immunodiagnosis ofAlveolar Echinococcosis inHumansbyan Enzyme-Linked Immunosorbent Assay UsingtheEm2Plus Antigen

Johannes Eckert
TL;DR: The Em2plus ELISA proved to be the optimal diagnostic test with the highest diagnostic sensitivity, 97%, in serum samples from 140 patients with AE and an overall specificity of 99% for infections due to other Echinococcus and non-Echinococbus parasites.
Journal ArticleDOI

Molecular and immunological diagnosis of echinococcosis.

Bruno Gottstein
- 01 Jul 1992 - 
TL;DR: Diagnosis is based on imaging techniques coupled with immunodiagnostic procedures and the discrimination of serum immunoglobulin isotype activity to various Echinococcus antigens in both cystic and alveolar echinococcosis have been suggested for diagnostic purposes as well as for monitoring patients after treatment.
Journal ArticleDOI

Is High Prevalence of Echinococcus multilocularis in Wild and Domestic Animals Associated with Disease Incidence in Humans

Bruno Gottstein, +7 more
- 01 May 2001 - 
TL;DR: The presence of infected domestic carnivores can increase E. multilocularis exposure risk in humans, and comparative statistical analyses of seroprevalence and clinical incidence showed an increase in Em2-seropreavalence from 1986 and 1996-97 but no increase in clinical incidence of alveolar hydatid disease.
Journal ArticleDOI

Combined ultrasound and serologic screening for hepatic alveolar echinococcosis in central China.

Gitte Bartholomot, +8 more
- 01 Jan 2002 - 
TL;DR: The prevalence of AE in humans in 2 districts of south Gansu Province, China is estimated by use of ultrasound and Echinococcus serology and serologies were positive in 77 (96%) of 80 of patients who had lesions typical of progressive AE.
References
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Journal ArticleDOI

Chemotherapy for larval echinococcosis in animals and humans: report of a workshop

Peter M. Schantz, +2 more
- 01 Jan 1982 - 
TL;DR: Treatment of human patients infected with E. granulosus is followed by subjective improvement in most, and evidence of regression of cysts in some; in other patients, cysts continue to grow or have been proven viable even after several months of high-dose mebendazole therapy.
Journal ArticleDOI

Spontaneous death of Echinococcus multilocularis: cases diagnosed serologically (by Em2 ELISA) and clinical significance.

Robert L. Rausch, +3 more
- 01 May 1987 - 
TL;DR: The findings show that the Em2 ELISA may be positive in patients having lesions of AHD in which the etiologic agent is no longer viable, in agreement with the clinical impression that the cestode was dead.
Journal ArticleDOI

Serological differentiation between Echinococcus granulosus and E. multilocularis infections in man.

Bruno Gottstein, +2 more
- 01 Jan 1983 - 
TL;DR: An enzyme-linked immunosorbent assay (ELISA) was adapted for the serological differential diagnosis of cystic or alveolar echinococcosis in man caused by Echinococcus granulosus or E. multilocularis respectively.
Journal ArticleDOI

Purification and characterization of a specific antigen from Echinococcus multilocularis.

Bruno Gottstein
- 01 May 1985 - 
TL;DR: A polypeptide (Em2a) purified by affinity chromatography from the Echinococcus multilocularis metacestode showed a high degree of purity as assayed by SDS‐PAGE and analytical isoelectrical focusing and a minor contamination with host albumin was revealed.
Journal ArticleDOI

Determination of parasite-specific immunoglobulins using the ELISA in patients with echinococcosis treated with mebendazole

Bruno Gottstein, +2 more
- 01 Jan 1984 - 
TL;DR: Parasite-specific immunoglobulin classes were determined by the ELISA in sera of 19 patients with cystic or alveolar echinococcosis before and after 2 years of continuous treatment with mebendazole at daily doses of 35–50 mg/kg body weight.
Related Papers (5)

Spontaneous death of Echinococcus multilocularis: cases diagnosed serologically (by Em2 ELISA) and clinical significance.

Robert L. Rausch, +3 more
- 01 May 1987 - 

Serological differentiation between Echinococcus granulosus and E. multilocularis infections in man.

Bruno Gottstein, +2 more
- 01 Jan 1983 - 

Seroepidemiologic screening of Echinococcus multilocularis infection in a European area endemic for alveolar echinococcosis

Solange Bresson-Hadni, +9 more
- 01 Dec 1994 - 

Improved primary immunodiagnosis of alveolar echinococcosis in humans by an enzyme-linked immunosorbent assay using the Em2plus antigen.

Bruno Gottstein, +3 more
- 01 Feb 1993 - 

Purification and characterization of a specific antigen from Echinococcus multilocularis.

Bruno Gottstein
- 01 May 1985 - 
Frequently Asked Questions (1)
Q1. What are the contributions in "Sero-epidemiological survey for alveolar echinococcosis (by em2-elisa) of blood donors in an endemic area of switzerland" ?

Gottstein et al. this paper compared a highly species-specific antigen ( Em2 ) and a commonly used E. granulosus hydatid fluid antigen ( EgHF ) for their suitability in seroepidemiology.