scispace - formally typeset
Open AccessJournal ArticleDOI

Sero-epidemiological survey for alveolar echinococcosis (by Em2-ELISA) of blood donors in an endemic area of Switzerland

Bruno Gottstein, +7 more
- 01 Nov 1987 - 
- Vol. 81, Iss: 6, pp 960-964
TLDR
Sera from 17,166 blood donors living in 10 cantons of northern Switzerland in an area endemic for Echinococcus multilocularis were investigated by serological survey for alveolar echinococcosis, finding 2 asymptomatic clinical cases of AE within this population of blood donors.
Abstract: 
Sera from 17 166 blood donors living in 10 cantons of northern Switzerland in an area endemic for Echinococcus multilocularis were investigated by serological survey for alveolar echinococcosis (AE). A highly species-specific antigen (Em2) and a commonly used E. granulosus hydatid fluid antigen (EgHF) were compared for their suitability in seroepidemiology. EgHF showed a degree of nonspecificity which did not allow direct detection of AE cases. Antibody reaction with Em2 resulted in the detection of 2 asymptomatic clinical cases of AE (seroprevalence 0·01%) within this population of blood donors. A further 4 persons were positive in Em2-ELISA. These 4 persons had negative imaging studies and will be followed serologically and clinically.

read more

Citations
More filters
Journal ArticleDOI

Differential immunodiagnosis between cystic hydatid disease and other cross-reactive pathologies

D Poretti, +7 more
- 01 Feb 1999 - 
TL;DR: Immunoblotting provided a 99% discrimination between seropositive pre-operative cystic hydatid disease cases and cross-reactive non-cestode parasitic infections or malignancies.
Journal ArticleDOI

Application of ultrasound in diagnosis, treatment, epidemiology, public health and control of Echinococcus granulosus and E. multilocularis.

Calum N. L. Macpherson, +2 more
- 01 Oct 2003 - 
TL;DR: The application of US in field and clinical settings has led to a better understanding of the natural history of CE and AE and to the development of a WHO standardized classification of cyst types for CE.
Journal ArticleDOI

Alveolar echinococcosis of the liver in a six-year-old girl with acquired immunodeficiency syndrome.

Michaela Sailer, +5 more
- 01 Feb 1997 - 
TL;DR: The present case indicates that a marked immunodeficiency caused by AIDS may substantially contribute to the rapid proliferation and early manifestation of the disease.
Journal ArticleDOI

Primary disease recurrence after liver transplantation for alveolar echinococcosis: long-term evaluation in 15 patients.

Solange Bresson-Hadni, +12 more
- 01 Oct 1999 - 
TL;DR: Primary disease recurrence is not rare after LT for AE, but an extensive extrahepatic radiological check‐up has to be performed before LT, and anti‐Eg immunoglobulin G follow‐up is the most useful test for early diagnosis of parasite recurrence.
Journal ArticleDOI

An epidemiologic survey of human alveolar echinococcosis in southwestern Germany. Römerstein Study Group.

Thomas Romig, +9 more
- 01 Oct 1999 - 
TL;DR: If the prevalence of 40/100,000 for active cases would be representative for the rural population in high endemicity areas, the current number of AE cases in southwestern Germany is considerably higher than previously suspected.
References
More filters
Journal ArticleDOI

Chemotherapy for larval echinococcosis in animals and humans: report of a workshop

Peter M. Schantz, +2 more
- 01 Jan 1982 - 
TL;DR: Treatment of human patients infected with E. granulosus is followed by subjective improvement in most, and evidence of regression of cysts in some; in other patients, cysts continue to grow or have been proven viable even after several months of high-dose mebendazole therapy.
Journal ArticleDOI

Spontaneous death of Echinococcus multilocularis: cases diagnosed serologically (by Em2 ELISA) and clinical significance.

Robert L. Rausch, +3 more
- 01 May 1987 - 
TL;DR: The findings show that the Em2 ELISA may be positive in patients having lesions of AHD in which the etiologic agent is no longer viable, in agreement with the clinical impression that the cestode was dead.
Journal ArticleDOI

Serological differentiation between Echinococcus granulosus and E. multilocularis infections in man.

Bruno Gottstein, +2 more
- 01 Jan 1983 - 
TL;DR: An enzyme-linked immunosorbent assay (ELISA) was adapted for the serological differential diagnosis of cystic or alveolar echinococcosis in man caused by Echinococcus granulosus or E. multilocularis respectively.
Journal ArticleDOI

Purification and characterization of a specific antigen from Echinococcus multilocularis.

Bruno Gottstein
- 01 May 1985 - 
TL;DR: A polypeptide (Em2a) purified by affinity chromatography from the Echinococcus multilocularis metacestode showed a high degree of purity as assayed by SDS‐PAGE and analytical isoelectrical focusing and a minor contamination with host albumin was revealed.
Journal ArticleDOI

Determination of parasite-specific immunoglobulins using the ELISA in patients with echinococcosis treated with mebendazole

Bruno Gottstein, +2 more
- 01 Jan 1984 - 
TL;DR: Parasite-specific immunoglobulin classes were determined by the ELISA in sera of 19 patients with cystic or alveolar echinococcosis before and after 2 years of continuous treatment with mebendazole at daily doses of 35–50 mg/kg body weight.
Related Papers (5)

Spontaneous death of Echinococcus multilocularis: cases diagnosed serologically (by Em2 ELISA) and clinical significance.

Robert L. Rausch, +3 more
- 01 May 1987 - 

Serological differentiation between Echinococcus granulosus and E. multilocularis infections in man.

Bruno Gottstein, +2 more
- 01 Jan 1983 - 

Seroepidemiologic screening of Echinococcus multilocularis infection in a European area endemic for alveolar echinococcosis

Solange Bresson-Hadni, +9 more
- 01 Dec 1994 - 

Improved primary immunodiagnosis of alveolar echinococcosis in humans by an enzyme-linked immunosorbent assay using the Em2plus antigen.

Bruno Gottstein, +3 more
- 01 Feb 1993 - 

Purification and characterization of a specific antigen from Echinococcus multilocularis.

Bruno Gottstein
- 01 May 1985 - 
Frequently Asked Questions (1)
Q1. What are the contributions in "Sero-epidemiological survey for alveolar echinococcosis (by em2-elisa) of blood donors in an endemic area of switzerland" ?

Gottstein et al. this paper compared a highly species-specific antigen ( Em2 ) and a commonly used E. granulosus hydatid fluid antigen ( EgHF ) for their suitability in seroepidemiology.