Journal ArticleDOI
Serological screening for Echinococcus multilocularis infections with ELISA.
About:
This article is published in The Lancet.The article was published on 1985-05-11. It has received 40 citations till now. The article focuses on the topics: Echinococcus multilocularis.read more
Citations
More filters
Book ChapterDOI
Detection, screening and community epidemiology of taeniid cestode zoonoses: cystic echinococcosis, alveolar echinococcosis and neurocysticercosis.
TL;DR: The chapter addresses the problem of neurocysticercosis and epilepsy in developing countries particularly Latin America and its public health consequences in the USA and elsewhere.
Book ChapterDOI
Echinococcosis: Control and Prevention.
TL;DR: Challenges that remain for echinococcosis control include effective intervention in resource-poor communities, better availability of surveillance tools, optimal application of livestock vaccination, and management and ecology of dog and wildlife host populations.
Journal ArticleDOI
A large focus of alveolar echinococcosis in central China.
Philip S. Craig,Liu Deshan,Calum N. L. Macpherson,Shi Dazhong,D. Reynolds,Guy Barnish,Bruno Gottstein,Wang Zhirong +7 more
TL;DR: The high prevalence of human AE in this region of central China is most probably due to semi-domestic transmission of E multilocularis between wild rodents and dogs, together with the poverty and poor hygiene in these rural communities.
Journal ArticleDOI
Improved primary immunodiagnosis of alveolar echinococcosis in humans by an enzyme-linked immunosorbent assay using the Em2plus antigen.
Abstract: Alveolar echinococcosis (AE) in humans is generally a fatal disease when not diagnosed early enough to provide curative treatment such as radical surgery. Immunodiagnosis for early detection of AE was improved by the isolation of an affinity-purified metacestode Em2 antigen and by the synthesis of recombinant Echinococcus multilocularis antigen II/3-10. Both antigens were individually assessed by enzyme-linked immunosorbent assay (ELISA) and demonstrated high specificities and diagnostic sensitivities, although both missed approximately 4 to 11% of diagnostic cases of AE. To provide an optimal serodiagnostic test, we investigated the two purified antigens by using a test employing a mixture of both purified antigens (designated Em2plus antigen) in one assay. For comparative purposes, crude E. multilocularis and Echinococcus granulosus metacestode antigens were investigated as well. The Em2plus ELISA proved to be the optimal diagnostic test with the highest diagnostic sensitivity, 97%, in serum samples from 140 patients with AE and an overall specificity of 99% for infections due to other Echinococcus and non-Echinococcus parasites. The new test combination (Em2plus ELISA) is suggested for the serodiagnosis of AE in patients and for seroepidemiological surveys.
Improved Primary Immunodiagnosis ofAlveolar Echinococcosis inHumansbyan Enzyme-Linked Immunosorbent Assay UsingtheEm2Plus Antigen
TL;DR: The Em2plus ELISA proved to be the optimal diagnostic test with the highest diagnostic sensitivity, 97%, in serum samples from 140 patients with AE and an overall specificity of 99% for infections due to other Echinococcus and non-Echinococbus parasites.
References
More filters
Journal ArticleDOI
Serological differentiation between Echinococcus granulosus and E. multilocularis infections in man.
TL;DR: An enzyme-linked immunosorbent assay (ELISA) was adapted for the serological differential diagnosis of cystic or alveolar echinococcosis in man caused by Echinococcus granulosus or E. multilocularis respectively.
Journal ArticleDOI
Purification and characterization of a specific antigen from Echinococcus multilocularis.
TL;DR: A polypeptide (Em2a) purified by affinity chromatography from the Echinococcus multilocularis metacestode showed a high degree of purity as assayed by SDS‐PAGE and analytical isoelectrical focusing and a minor contamination with host albumin was revealed.
Journal ArticleDOI
Alveolar echinococcosis of the liver. Studies on 60 operated cases.
TL;DR: During the years 1936 through 1978, 60 cases received surgical intervention for alveolar echinococcosis of the liver, the re-sectability and operative mortality rate were 64.0% and 43.8% before 1968, but 54.3% and zero thereafter.
Journal ArticleDOI
Is alveolar hydatid disease of the liver incurable
TL;DR: The present data suggest that surgical cure of alveolar hydatid disease must be very rare.
Journal ArticleDOI
Serologic Tests for Diagnosis and Post-Treatment Evaluation of Patients with Alveolar Hydatid Disease (Echinococcus Multilocularis)
TL;DR: The indirect hemagglutination and immunoelectrophoresis tests were used for diagnosis and follow-up evaluation of 17 patients with alveolar hydatid disease caused by Echinococcus multilocularis, and antibody has persisted at high levels in non-resected patients treated continuously with high doses of mebendazole.