Journal ArticleDOI
Simple sequence repeat (SSR) markers reveal low levels of polymorphism between cotton (Gossypium hirsutum L.) cultivars
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Estimating the level of polymorphism detected with a set of commercially available SSR markers between and within a collection of cotton cultivars being used in breeding programs suggests that where polymorphisms exist, heterozygosity within cultivars is maintained by the breeding strategies adopted by many modern cotton breeders.Abstract:
Since their discovery in the 1980s microsatellite or simple sequence repeat (SSR) markers have been widely used in many species to generate relatively dense genetic maps or framework maps on which to anchor more abundant, but anonymous, markers such as amplified fragment length polymorphisms (AFLPs). They are typically highly polymorphic, robust, and often portable, particularly among different mapping populations or crosses and often to related species. They have been useful in species where low levels of genetic diversity limit the use of other markers. Cultivated cotton (Gossypium hirsutum L.) has a history of genetic bottlenecks that have considerably reduced its diversity, with the consequence that most molecular marker genetic linkage studies are done with inter-specific crosses. In this study we evaluated the potential for SSR markers to be used in marker-assisted selection (MAS) breeding in cotton by quantifying the level of polymorphism detected with a set of commercially available SSR markers between and within a collection of cotton cultivars being used in our breeding programs. Although the majority of these markers are polymorphic between the 2 tetraploid species of cotton, G. barbadense and G. hirsutum, they are not highly polymorphic (~5%) either among or within G. hirsutum cultivars. However, 6 of the 8 cultivars studied were found to be segregating for alleles of these SSR markers. This suggests that where polymorphisms exist, heterozygosity within cultivars is maintained by the breeding strategies adopted by many modern cotton breeders. Although SSRs clearly have utility in genetic studies using inter-specific crosses or in the introgression of wild germplasm, they will be more difficult to use for standard cotton breeding until greater numbers are available. The utility of some markers may be reduced in some breeding populations where heterozygosity remains in the parental material.read more
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Book ChapterDOI
Evolution and natural history of the cotton genus.
TL;DR: This framework facilitates insight into fundamental aspects of plant biology, provides the necessary underpinnings for effective utilization of cotton genetic resources, and guides exploration of the genomic basis of morphological diversity in the genus.
Journal ArticleDOI
Recent advances in cotton genomics.
TL;DR: Analysis of the current status of each of the genome research areas suggests that the areas of physical mapping, QTL fine mapping, genome sequencing, nonfiber and nonovule EST development, gene expression profiling, and association studies between gene expression and fiber trait performance should be emphasized currently and in near future to accelerate utilization of the genomics research achievements for enhancing cotton genetic improvement.
Journal ArticleDOI
Microsatellite diversity in tetraploid Gossypium germplasm: assembling a highly informative genotyping set of cotton SSRs
TL;DR: A series of 320 mapped simple sequence repeats (SSRs) have been used to screen the allelic diversity of tetraploid Gossypium species, and race yucatanense appeared as the most distant from cultivated genotypes, exhibiting the highest number of unique alleles.
Journal ArticleDOI
Linkage disequilibrium based association mapping of fiber quality traits in G. hirsutum L. variety germplasm
Ibrokhim Y. Abdurakhmonov,Sukumar Saha,J. N. Jenkins,Zabardast T. Buriev,Shukhrat E. Shermatov,Brain E. Scheffler,Alan E. Pepper,John Z. Yu,Russell J. Kohel,Abdusattor Abdukarimov +9 more
TL;DR: Results suggest linkage, selection, inbreeding, population stratification, and genetic drift as the potential LD-generating factors in cotton.
Journal ArticleDOI
Genetic structure, linkage disequilibrium and association mapping of Verticillium wilt resistance in elite cotton (Gossypium hirsutum L.) germplasm population.
TL;DR: The LD level for linked markers is significantly higher than that for unlinked markers, suggesting that physical linkage strongly influences LD in this panel, and LD level was elevated when the panel was classified into groups and subgroups.
References
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Journal ArticleDOI
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TL;DR: The objectives of this research were to calculate polymorphism information content values of 51 low-copy DNA clones and use the resulting values to choose potential mapping parents and to identify Genotype pairs that would map more highly informative DNA clones compared with a population derived from the most polymorphic potential parents.
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Journal Article
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