Journal ArticleDOI
Size measurements on isolated rat heart cells using Coulter analysis and light scatter flow cytometry.
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Preliminary electronic gating experiments, using pulse height signals, suggest that signals derived from round cells could be eliminated entirely using a gating regime based on pulse width, which would enable flow cytometric measurements to be made on only the intact myocytes present in heterogeneous preparations.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1979-09-20. It has received 45 citations till now. The article focuses on the topics: Pulse duration & Population.read more
Citations
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Journal ArticleDOI
Regression of Cellular Hypertrophy After Left Ventricular Assist Device Support
TL;DR: These studies suggest that the regression of cellular hypertrophy is a major contributor to the "reverse remodeling" of the heart after LVAD implantation and may contribute to reduced wall stress and improved mechanical performance after LVad support.
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Surface:volume relationship in cardiac myocytes studied with confocal microscopy and membrane capacitance measurements: species-dependence and developmental effects
TL;DR: The data suggest that the ratio of surface area:volume of cardiac myocytes undergoes significant developmental changes and differs among mammalian species and the easily measurable parameters of cell membrane capacitance or the product of cell length and width provide reliable but species-dependent estimates for the volume of individual cells.
Surface:Volume Relationship inCardiac Myocytes Studied withConfocal Microscopy andMembraneCapacitance Measurements: Species- Dependence andDevelopmental Effects
Hiroshi Satoh,Lothar A. Blatter +1 more
TL;DR: In this article, the volume of cardiac myocytes of three different mammalian species was determined by three-dimensional volumerendering of the confocal images, and the calculated cell volumes were 30.4 ± 7.3pl(mean ± SD)inrabbits (n= 28), 30.9 ± 9.0pl(n= 23), and 34.4
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Distribution and three-dimensional structure of intercellular junctions in canine myocardium.
TL;DR: True "lateral" gap junctions do not exist in working ventricular myocytes and would not likely be able to withstand shear forces created by laterally sliding cells.
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The c-myc proto-oncogene regulates cardiac development in transgenic mice.
TL;DR: It is indicated that constitutive expression of c-myc mRNA in the heart during development results in enhanced hyperplastic growth and a regulatory role for this proto-oncogene in cardiac myogenesis is suggested.
References
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Journal ArticleDOI
A rapid technique for the isolation and purification of adult cardiac muscle cells having respiratory control and a tolerance to calcium
T. Powell,V.W. Twist +1 more
TL;DR: The complete procedure is rapid and isolated myocytes beat, exclude vital stains, have conventional sub-cellular morphology, show tight respiratory coupling and also have a tolerance to external calcium not found in cells isolated by other perfusion techniques.
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Cell Sizing: A Light Scattering Photometer for Rapid Volume Determination
TL;DR: Volume distributions of mammalian cells and plastic microspheres agree with other independent determinations on small angle light scattering by spherical particles.
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Electrical counting and sizing of mammalian cells in suspension.
E.C. Gregg,K.David Steidley +1 more
TL;DR: It is found that most biological cells may be treated as dielectric particles whose capacitive effects are negligible and the current pulse may be made conductivity-independent when observed with a zero input impedance amplifier.
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Sizing particles with a Coulter counter.
TL;DR: A theorem is presented which permits a determination of the amplitude of the signal generated by the passage of a particle of arbitrary shape through a Coulter counter, which is directly proportional to the volume of the particle.
Journal ArticleDOI
Basic principles of electrical sizing of cells and particles and their realization in the new instrument "Metricell".
TL;DR: A new compact sizing instrument, "Metricell," equipped with a particle-independent electrical calibrating system, is described, and the absolute volume evaluation of particles has been studied with model particles in enlarged model orifices of different lengths.