Journal ArticleDOI
Solid‐Phase Synthesis of Oligodeoxynucleotides containing phosphoramidate internucleotide linkages and their specific chemical cleavage
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TLDR
Oligonuclecotides bearing phosphoramidate internucleotide linkages can be prepared chemically by standard solid-phase DNA synthesis and placed at will into specific positions within a given DNA fragment.Abstract:
Oligonuclecotides bearing phosphoramidate internucleotide linkages can be prepared chemically by standard solid-phase DNA synthesis. Thus, phosphoramidate internucleotide bonds can be placed at will into specific positions within a given DNA fragment. The backbone-modified DNA fragments prepared in this way are susceptible to a specific chemical cleavage.read more
Citations
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Patent
Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups
TL;DR: In this paper, a method for improving the hybridization properties of oligonucleotides and novel processes for preparing 3'-phosphorylated oligon nucleotides are presented, respectively.
Patent
Dna sequencing by mass spectrometry
TL;DR: In this article, a new method to sequence DNA is described, which assembles the sequence information by analysis of the nested fragments obtained by base-specific chain termination via their different molecular masses using mass spectrometers.
Patent
DNA diagnostics based on mass spectrometry
TL;DR: In this article, a mass spectrometer-based process for detecting nucleic acid sequences in a biological sample was proposed, which can be used to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity.
Journal ArticleDOI
MODIFIED OLIGONUCLEOTIDES: Synthesis and Strategy for Users
Sandeep Verma,Fritz Eckstein +1 more
TL;DR: The range, scope, and practical utility of chemically modified oligonucleotides that contain backbone, base, and sugar modifications to investigate the mechanism and stereochemical aspects of biochemical reactions are reviewed.
References
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Journal ArticleDOI
Polymer support oligonucleotide synthesis XVIII1.2): use of β-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product
TL;DR: Various 5'O-N-protected deoxynucleoside-3'-O-beta-cyanoethyl-N,N-dialkylamino-/N- morpholinophosphoramidites were prepared from beta-Cyanoethyl monochlorophosphors of N, N-dimethylamine, N, n-diisopropylamine and N-morpholine to provide stability in solution for automated DNA-synthesis.
Journal ArticleDOI
An investigation of several deoxynucleoside phosphoramidites useful for synthesizing deoxyoligonucleotides
L.J. McBride,Marvin H. Caruthers +1 more
TL;DR: In this article, various deoxynucleoside N,N-dialkylaminomethoxyphosphines were examined for stability and reactivity in deoxyoligonucleotide synthesis.
Journal ArticleDOI
A Simple and Effective Chemical Phosphorylation Procedure for Biomolecules
Willi Bannwarth,Arnold Trzeciak +1 more
TL;DR: A general chemical phosphorylation method based on P(III) chemistry has been developed and is demonstrated for the phosphorylations of oligonucleotides, directly after their synthesis on a solid support, and for the O-phosphorylation of serine, threonine, and tyrosine as well as for a serine-containing peptide.
Journal ArticleDOI
Hindered dialkylamino nucleoside phosphite reagents in the synthesis of two DNA 51-mers
Journal ArticleDOI
In situ activation of bis-dialkylaminophosphines—a new method for synthesizing deoxyoligonucleotides on polymer supports
TL;DR: These phosphoramidites generated in situ lead to the direct synthesis of deoxyoligonucleotides on polymer supports.
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