Journal ArticleDOI
Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee ( Coffea canephora P. ex Fr.)
TLDR
Somatic embryogenesis was achieved in protoplast cultures of coffee by transferring microcalli from cell suspension-derived somatic embryos to a medium lacking growth regulators, which resulted in the formation of globular embryos.Abstract:
Somatic embryogenesis was achieved in protoplast cultures of coffee. Protoplasts were isolated from cell suspension-derived somatic embryos of Coffea canephora. After repeated subculture in a medium supplemented with 0.5 mg/l of each of kinetin, 2,4-dichlorophenoxyacetic acid (2,4-D), and naphthaleneacetic acid (NAA), microcalli developed. Transfer of these microcalli to a medium lacking growth regulators resulted in the formation of globular embryos. Upon subculture without growth regulators they grew to well-differentiated embryos, Eventually some of them developed to plantlets which were transferred to the greenhouse for further observation.read more
Citations
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Journal ArticleDOI
High frequency somatic embryogenesis in Coffea canephora: induction conditions and histological evolution
TL;DR: Leaf explants of Coffea canephora produced a friable yellow callus when they were cultured on a conditioning basal medium and this calus could be maintained by means of regular subcultures or it could give rise to somatic embryos depending on the culture medium.
Book ChapterDOI
Somatic Embryogenesis in Woody Plants
TL;DR: The description for herbaceous plants “with a relatively short-lived aboveground stem that is comparatively thin, soft, and nonwoody” contrasts with the description for timber plants, which are regarded as herbaceous here.
Journal ArticleDOI
Echinacea biotechnology: Challenges and opportunities
TL;DR: An overview of the recent developments in in vitro technologies and challenges that remain in the Echinacea biotechnology is provided.
Journal ArticleDOI
Progress in Plant Protoplast Research
TL;DR: The progress made during the past four years is indeed impressive and demonstrates that the interest in and the potential of plant protoplast research is far from slowing down.
Journal ArticleDOI
Plant regeneration from leaf mesophyll protoplasts of the tropical woody plant, passionfruit (Passiflora edulis fv flavicarpa Degener.): the importance of the antibiotic cefotaxime in the culture medium.
F. B. d'Utra Vaz,A. V. P. dos Santos,G. Manders,Edward C. Cocking,Michael R. Davey,J. B. Power +5 more
TL;DR: The ability to regenerate plants from protoplasts of passionfruit is discussed in relation to the application of somatic cell techniques for the genetic improvement of this economically important tropical woody plant.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Journal ArticleDOI
Plant regeneration from Citrus protoplasts: Variability in methodological requirements among cultivars and species.
TL;DR: The establishment of eight new protoplast systems in Citrus paves the way for cell genetics studies and for novel breeding approaches in these economically important orchard trees.
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A revised medium for rapid growth and bio assays with tobacco tissue cultures
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