Journal ArticleDOI
Spectrophotometric determination of phosphate esters in the presence and absence of orthophosphate.
E.M. Bartlett,D.H. Lewis +1 more
Reads0
Chats0
TLDR
A simple modified molybdovanadate method for the simultaneous assay of orthophosphate and some organic phosphates, especially carbohydrate esters, is described, applicable to the study of specificity of phosphatases and assay of individual esters after chromatographic separation.About:
This article is published in Analytical Biochemistry.The article was published on 1970-07-01. It has received 149 citations till now. The article focuses on the topics: Phosphate.read more
Citations
More filters
Journal ArticleDOI
Quantitative determination of phospholipid compositions by ESI-MS: effects of acyl chain length, unsaturation, and lipid concentration on instrument response.
TL;DR: This study indicates that quantitative compositional data can be obtained with ESI-MS, provided that proper attention is paid to experimental details, particularly the choice of internal standards.
Journal ArticleDOI
Automated quantitative analysis of complex lipidomes by liquid chromatography/mass spectrometry.
TL;DR: This work has developed an automated method that allows unattended identification and quantification of lipid molecular species of all the major lipid classes from a two-dimensional chromatographic/mass spectrometric data set and makes high-throughput lipidomics feasible in biology, biotechnology, and medicine.
Journal ArticleDOI
Soil isolates of Pseudomonas spp. that utilize inositol phosphates
Alan Richardson,P. A. Hadobas +1 more
TL;DR: Four unique organisms were selected and characterized for their ability to specifically utilize IHP and exhibited marked phytase activity and liberated up to 81% of the phosphate from IHP either in the absence or presence of arabinose as an additional C source.
Journal ArticleDOI
Both Sphingomyelin Synthases SMS1 and SMS2 Are Required for Sphingomyelin Homeostasis and Growth in Human HeLa Cells
Fikadu G. Tafesse,Klazien Huitema,Martin Hermansson,Seleéne van der Poel,Joep van den Dikkenberg,Andreas Uphoff,Pentti Somerharju,Joost C. M. Holthuis +7 more
TL;DR: It is shown that SMS1 and SMS2 are co-expressed in a variety of cell types and function as the key Golgi- and plasma membrane-associated SM synthases in human cervical carcinoma HeLa cells, respectively, indicating that the biological role of SM synthase goes beyond formation of SM.
Journal ArticleDOI
High-Throughput Analysis of Sphingosine 1-Phosphate, Sphinganine 1-Phosphate, and Lysophosphatidic Acid in Plasma Samples by Liquid Chromatography–Tandem Mass Spectrometry
TL;DR: The authors' validated LC-MS/MS methodology for quantifying LPA, S1P, and SA1P features simple sample preparation and short analysis times, therefore providing a valuable tool for diagnostic evaluation of these lysophospholipids as biomarkers.
References
More filters
Journal ArticleDOI
Determination of phosphate by differential spectrophotometry.
Allen. Gee,V. R. Deitz +1 more
Journal ArticleDOI
Photometric Determination of Phosphorus as Molybdovanadophosphoric Acid
Journal ArticleDOI
Spectrophotometric Determination of Phosphorus as Molybdovanadophosphoric Acid
K. P. Quinlan,M. A. DeSesa +1 more
Journal ArticleDOI
Determination of inorganic phosphate in the presence of labile organic phosphates.
Rehana Parvin,Roberts A. Smith +1 more
TL;DR: An operationally simple and rapid procedure of orthophosphate determination, based on the formation of a phosphomolybdovanadate complex, followed by its subsequent extraction into butanol, is described.