Journal ArticleDOI
Spectrophotometric method for the simultaneous determination of proteins and amino acids with p-benzoquinone
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TLDR
In this article, a spectrophotometric method for the simultaneous determination of proteins and total amino acids in mixtures is described, based on the observation that the products of the reaction between p -benzoquinone (PBQ) and proteins show an absorbance band at about 350 nm, whereas for most of the essential amino acids the absorbance bands are at about 440 nm.About:
This article is published in Analytica Chimica Acta.The article was published on 1993-05-15. It has received 20 citations till now. The article focuses on the topics: Amino acid & Absorbance.read more
Citations
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Journal ArticleDOI
Determination of total proteins in cow milk powder samples: a comparative study between the Kjeldahl method and spectrophotometric methods.
TL;DR: In this article, a comparative study between the Kjeldahl method and several spectrophotometric methods was carried out for the determination of total proteins in a range of milk powder samples.
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Spectrophotometric determination of cysteine and/or carbocysteine in a mixture of amino acids, shampoo, and pharmaceutical products using p-benzoquinone.
TL;DR: The results showed the reaction between p-benzoquinone and Cys occurs through the sulfhydryl group, and a recovery of about 100% is observed for both Cys and carboCys, when the method is applied to determine Cys in a mixture of amino acids resembling blood plasma, shampoo, and pill food.
Journal ArticleDOI
Colorimetric determination of gabapentin in pharmaceutical formulation.
TL;DR: Three accurate, simple and precise colorimetric methods for the determination of gabapentin in capsules are developed revealing high accuracy and good precision.
Journal ArticleDOI
The role of the thiol group in protein modification with methylglyoxal
TL;DR: The importance of the reaction of the Cys thiol group with methylglyoxal in protein modification, the competitiveness of this reaction with those of amino and guanidine groups, the time course of these reactions and their role and contribution to protein cross-linking were investigated.
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Colorimetric Determination of Benzocaine, Lignocaine and Procaine Hydrochlorides in Pure Form and in Pharmaceutical Formulations Using p-Benzoquinone
TL;DR: The proposed procedure of analysis is as accurate as the British Pharmacopoeial method (2003) and was successfully used for the determination of those drugs in the presence of their degradation products, additives and excipients, which were normally encountered in pharmaceutical formulations.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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Determination of serum proteins by means of the biuret reaction.
TL;DR: An investigation of the biochemical changes following experimental liver injury felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum and began with Kingsley’s biuret procedure.
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An absolute method for protein determination based on difference in absorbance at 235 and 280 nm
TL;DR: The use of difference in absorbance between 235 and 280 nm for determination of protein concentration is reported.