Patent
Stable isotope metabolic labeling for analysis of biopolymers
B. Robert Franza,Yvan Rochon +1 more
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TLDR
In this article, the rate of synthesis of biopolymer synthesis and degradation in cells, tissues, or cell-free systems using monomer which has been labeled with a stable isotope.Abstract:
Methods for the determination of the rate of synthesis of biopolymer synthesis and degradation in cells, tissues, or cell-free systems using monomer which has been labeled with a stable isotope are provided. Further, the present invention provides methods for the determination or identification of an unknown biopolymer and for the identification of an unknown cell type, a physiological state of a cell or tissue. Also, the present invention provides a database of descriptors which can be used to define an organism, tissue type, cell type, and the like, and which database can be used in conjunction with other public and private databases to identify or characterize an organism, tissue type, cell type, state of differentiation, or physiologic state of an organism, or tissue or cell sample.read more
Citations
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Current trends in quantitative proteomics
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The revolution and evolution of shotgun proteomics for large-scale proteome analysis.
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Patent
Method for identifying or characterizing properties of polymeric units
TL;DR: In this article, the authors proposed methods of identifying and characterizing properties of polymers to provide information about the polymer such as the charge of the polymer, the number and types or characteristics of units of the polymers and the sequence of thepolymers.
Patent
Methods for comparing relative flux rates of two or more biological molecules in vivo through a single protocol
TL;DR: In this article, a technique for measuring and comparing relative molecular flux rates of different biological molecules by administering isotope-labeled water to one or more tissues or individuals and comparing the molecular flux rate of two or more biological molecules, including biological molecules in different chemical classes.
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Molecular flux rates through critical pathways measured by stable isotope labeling in vivo, as biomarkers of drug action and disease activity
TL;DR: In this paper, stable isotope-labeled substrate molecules are administered to a subject and the label is incorporated into targeted molecules in a manner that reveals molecular flux rates through one or more metabolic pathways of interest.
References
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Journal ArticleDOI
An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.
TL;DR: The approach described in this manuscript provides a convenient method to interpret tandem mass spectra with known sequences in a protein database.
Journal ArticleDOI
Mass isotopomer analysis: Theoretical and practical considerations
TL;DR: A theory of mass isotopomer analysis based on the well-known principle of isotope dilution mass spectrometry is reviewed and examples of the application of this theory to determine the spectrum of the trimethylsilyl derivative of the 'pure unlabeled' or mononuclidic cholesterol are provided.
Patent
Use of mass spectrometry fragmentation patterns of peptides to identify amino acid sequences in databases
John R. Yates,Jimmy K. Eng +1 more
TL;DR: In this paper, a method for correlating a peptide fragment mass spectrum with amino acid sequences derived from a database is provided, where the peptide is analyzed by a tandem mass spectrometer to yield a peptides fragment spectrum, and a plurality of fragments of the sequence are identified and the masses and m/z ratios of the fragments are predicted and used to form a predicted mass spectrum.
Journal ArticleDOI
Regulation of leucine metabolism in man: a stable isotope study.
Dwight E. Matthews,Dennis M. Bier,Michael J. Rennie,R. H. T. Edwards,D. Halliday,David J. Millward,G. A. Clugston +6 more
TL;DR: Leucine transamination was found to be operating several times faster than the keto acid decarboxylation and to be of equal magnitude in adult human males under two different dietary conditions, post absorbing and fed.
Journal ArticleDOI
The SWISS-PROT protein sequence data bank, recent developments
TL;DR: The SWISS-PROT database distinguishes itself from other protein sequence databases by three distinct criteria: protein sequence quality, consistency and utility.