Journal ArticleDOI
Stimulation of the respiration of polymorphonuclear leucocytes by phospholipase C.
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TLDR
Polymorphonuclear leucocytes treated in vitro with phospholipase C exhibit a marked increase of the respiration, which is insensitive to KCN, and of the catabolism of glucose through the hexose monophosphate shunt, similar to those occuring in phagocytosing polymorphonuclearLeucocytes.About:
This article is published in Life Sciences.The article was published on 1970-08-01. It has received 50 citations till now. The article focuses on the topics: Phospholipase & Phospholipase C.read more
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Localization of NADH oxidase on the surface of human polymorphonuclear leukocytes by a new cytochemical method.
TL;DR: These findings, combined with oxygen consumption studies on resting and stimulated PMN in the presence or absence of NADH, indicate that NADH oxidase is a surface enzyme in human PMN, which is internalized duringphagocytosis and retains its peroxide-generating capacity within the phagocytic vacuole.
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The superoxide-forming enzymatic system of phagocytes
TL;DR: The triggering mechanism of the respiratory burst is discussed, with particular reference to the stimulus-response coupling and the final modification(s) of the oxidase (phosphorylation, assembly, change of lipid environment, etc.) which are involved in its activation.
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Enzymatic basis of metabolic stimulation in leucocytes during phagocytosis: the role of activated NADPH oxidase.
TL;DR: The metabolic stimulation of PMN leucocytes by phagocytosis is supported by changes in kinetic properties of NADPH oxidase, which induces a 3-fold increase of the intracellular NADP + NADPH ratio, without affecting the NAD+ NADH ratio.
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Clostridium perfringens alpha-toxin : Characterization and mode of action
TL;DR: Current knowledge of the characteristics and mode of action of alpha-toxin is summarized to provide a better understanding of the role of alpha -toxin in tissue damage in gas gangrene.
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Effects of fatty acids on superoxide radical generation in leukocytes.
TL;DR: Acetylated ferricytochrome c was employed for the detection of superoxide radicals (O-2) generated both in intact cells and in subcellular fractions of leukocytes, suggesting a correlation between the formation of O-2 and the hydrophobic interaction of fatty acids with the leukocyte plasma membrane.
References
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Journal ArticleDOI
A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
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The biochemical basis of phagocytosis. I. Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes.
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Biochemical Aspects of Phagocytosis
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The extra respiration of phagocytosis
C. W. Baldridge,R. W. Gerard +1 more
TL;DR: As other cells increase their energy turnover during activity, it might be anticipated that the respiration of leucocytes would increase during active phagocytic ingestion-unless the entire process is controlled by physical tensions.
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Sarcoplasmic Reticulum III. THE ROLE OF PHOSPHOLIPIDS IN THE ADENOSINE TRIPHOSPHATASE ACTIVITY AND Ca++ TRANSPORT
TL;DR: Treatment of skeletal muscle microsomes with phospholipase C inhibits the adenosine triphosphatase activity and calcium transport, parallel with the selective hydrolysis of membrane lecithin, and is accompanied by tight binding ofospholipids to the microsomal membrane.