Journal ArticleDOI
Structural basis of specificity in tetrameric Kluyveromyces lactis β-galactosidase.
Ángel Pereira-Rodríguez,Rafael Fernandez-Leiro,Rafael Fernandez-Leiro,M. Isabel González-Siso,M. Esperanza Cerdán,Manuel Becerra,Julia Sanz-Aparicio +6 more
TLDR
This is the first X-ray crystal structure for a β-galactosidase used in food processing and it shows the tetrameric enzyme is a dimer of dimers, with higher dissociation energy for the dimers than for its assembly.About:
This article is published in Journal of Structural Biology.The article was published on 2012-02-01. It has received 83 citations till now. The article focuses on the topics: Kluyveromyces lactis & Active site.read more
Citations
More filters
Journal ArticleDOI
N-acetylgalatosamine-Mediated Regulation of the aga Operon by AgaR in Streptococcus pneumoniae.
TL;DR: The transcriptome and RT-PCR data suggest the role of AgaR as a transcriptional repressor of the aga operon, which is putatively involved in NAGa transport and utilization, in the presence of NAGA.
Journal ArticleDOI
High stability of immobilized β-D-galactosidase for lactose hydrolysis and galactooligosaccharides synthesis.
Manuela P. Klein,Lucas Prestes Fallavena,Jéssie da Natividade Schöffer,Marco Antônio Záchia Ayub,Rafael C. Rodrigues,Jorge Luiz Ninow,Plinho Francisco Hertz +6 more
TL;DR: β-D-Galactosidase from Kluyveromyces lactis was immobilized on glutaraldehyde-activated chitosan and used in a packed-bed reactor for the continuous hydrolysis of lactose and the synthesis of galactooligosaccharides (GOS).
Journal ArticleDOI
Transgalactosylation and hydrolytic activities of commercial preparations of β-galactosidase for the synthesis of prebiotic carbohydrates.
TL;DR: The balance between transgalactosylation and hydrolytic activities of β-Galactosidases could be tuned by changing the concentration of galactose donor, as reflected by the Michaelis constants obtained in the synthesis of galacts, fructosyl-galacto-oligosaccharides and lactulose.
Journal ArticleDOI
Reaction kinetics and galactooligosaccharide product profiles of the β-galactosidases from Bacillus circulans, Kluyveromyces lactis and Aspergillus oryzae.
TL;DR: A detailed analysis of the clearly different GOS profiles of the commercial β-galactosidases from Bacillus circulans, Kluyveromyces lactis and Aspergillus oryzae is reported.
Journal ArticleDOI
Synthesis of novel bioactive lactose‐derived oligosaccharides by microbial glycoside hydrolases
TL;DR: This review presents an overview on the most recent interesting approaches to synthesize lactose‐derived oligosaccharides with potential prebiotics activity paying special focus on the microbial glycoside hydrolases that can be effectively employed to obtain these prebiotic compounds.
References
More filters
Journal ArticleDOI
Coot: model-building tools for molecular graphics.
Paul Emsley,Kevin Cowtan +1 more
TL;DR: CCP4mg is a project that aims to provide a general-purpose tool for structural biologists, providing tools for X-ray structure solution, structure comparison and analysis, and publication-quality graphics.
Journal ArticleDOI
PROCHECK: a program to check the stereochemical quality of protein structures
TL;DR: The PROCHECK suite of programs as mentioned in this paper provides a detailed check on the stereochemistry of a protein structure and provides an assessment of the overall quality of the structure as compared with well refined structures of the same resolution.
Journal ArticleDOI
The CCP4 suite: programs for protein crystallography
TL;DR: The CCP4 (Collaborative Computational Project, number 4) program suite is a collection of programs and associated data and subroutine libraries which can be used for macromolecular structure determination by X-ray crystallography.
Journal ArticleDOI
Refinement of macromolecular structures by the maximum-likelihood method.
TL;DR: The likelihood function for macromolecular structures is extended to include prior phase information and experimental standard uncertainties and the results derived are consistently better than those obtained from least-squares refinement.
Journal ArticleDOI
The Pfam protein families database
Marco Punta,Penny Coggill,Ruth Y. Eberhardt,Jaina Mistry,John Tate,Chris Boursnell,Ningze Pang,Kristoffer Forslund,Goran Ceric,Jody Clements,Andreas Heger,Liisa Holm,Erik L. L. Sonnhammer,Sean R. Eddy,Alex Bateman,Robert D. Finn +15 more
TL;DR: The definition and use of family-specific, manually curated gathering thresholds are explained and some of the features of domains of unknown function (also known as DUFs) are discussed, which constitute a rapidly growing class of families within Pfam.