Showing papers in "Enzyme and Microbial Technology in 2015"

TL;DR: The results confirm that the lipases immobilized on octyl agarose presented their open form stabilized while the covalent preparation maintains a closing/opening equilibrium that may be modulated by altering the medium.

TL;DR: This work describes a tandem-yeast expression system for UPO engineering and large scale production that ensures the engineering of UPOs to use them in future industrial applications as well as large scaleProduction.

TL;DR: The comparison of the biosensor performance with reported sensors reveals the significant improvement in overall sensor performance and the fabricated biosensor exhibited appreciable stability, repeatability, reproducibility and practicality.

TL;DR: New insights are provided into the role of accessory enzymes, which can further provide a useful reference for the rational customization of cellulase cocktails in order to realize an efficient conversion of natural lignocellulosic substrates.

TL;DR: This work shows that the recycling strategy must be customized for each particular formulation, since the enzymes found e.g. in Cellic and Celluclast bear quite different behaviour regarding the solid-liquid distribution, stability and cellulose and lignin affinity.

TL;DR: The interaction between cellulases and xylanases seems to be an effective alternative to reduce enzyme loading in the saccharification process in the second-generation ethanol industry.

TL;DR: It is claimed that the drastic increases in activities of HNF are associated with to high local HRP concentration in nanoscale dimension, appropriate HRP conformation, less mass transfer limitations, and role of Fe(2+) ion as an activator for HRP.

TL;DR: The balance between transgalactosylation and hydrolytic activities of β-Galactosidases could be tuned by changing the concentration of galactose donor, as reflected by the Michaelis constants obtained in the synthesis of galacts, fructosyl-galacto-oligosaccharides and lactulose.

TL;DR: Using p-nitrophenyl butyrate (p-NPB) and methyl phenylacetate, activity always improved by the blocking step, whatever the blocking reagent, while with methyl mandelate or ethyl hexanoate not always the blocking presented a positive effect.

TL;DR: In this paper, Escherichia coli was engineered for the production of caffeic acid using tyrosine as the initial precursor of the pathway and the highest production obtained was 2.62mM (472mg/L).

TL;DR: In this article, the effects of various compounds associated with lignocellulosic hydrolysates on individual and combinations of cellulases were investigated, and it was shown that inhibited lignin residues had little or no inhibitory effect on enzymes.

TL;DR: The inactivation of MW-treated HRP was related to the loss of tertiary structure, indicating changes around the tryptophan environment.

TL;DR: Ethanol has the potential to be used as an effective reagent to induce astaxanthin accumulation in H. pluvialis and shows that ethanol greatly enhanced transcription of the isopentenyl diphosphate (IPP) isomerase genes (ipi-1 and ipi-2), which were responsible for isomerization reaction of IPP and dimethylallyl diph phosphate (DMAPP).

TL;DR: It was observed that the GH family classification of an enzyme affects its substrate specificity and synergistic interactions with other glycoside hydrolases from different families (more so than its EC classification).

TL;DR: It appears that the facile physical adsorption of CRL onto F-MWCNTs has improved the activity and stability of C RL as well as served as an alternative method for the synthesis of geranyl propionate.

TL;DR: The properties of GH61 are investigated and the synergistic activity of GtGH61 with xylanase or cellulase showed efficient bioconversion rates of 56 and 174% in pretreated kenaf (Hibiscus cannabinus L.) and oak (Quercus spp.), respectively.

TL;DR: Structural change analysis revealed that the increase in thermostability was most probably caused by a combination of lower stability free-energy and higher hydrophobicity of E518I, more hydrogen bonds of S662R, and higher rigidity of Q706P compared with the WT.

TL;DR: A novel thermostable mannanase from a newly isolated Bacillus pumilus GBSW19 has been identified, expressed, purified and characterized and can be utilized to generate high value-added oligosaccharides from locust bean gum.

TL;DR: The results suggested that the assay developed in this study of Gram's iodine could be useful to predict the potential of microorganisms to produce alginate lyase.

TL;DR: A large-scale synthesis of lacto-N-tetraose was demonstrated using fed-batch cultivation with galactose as sole energy and carbon source and the influence of the carbon source on the formation of lactosaccharide structures and on the intracellular availability of the glycosyltransferase substrates was investigated.

TL;DR: The results collectively indicated GPPS as an important regulation point in balancing a recombinant geraniol synthesis pathway and the GPPS-based regulation approach could be applicable for optimizing microbial production of other monoterpenes.

TL;DR: The two-step recycling pathway was found to be the most promising for its integration with E. coli metabolism, more efficient, more sustainable and cost-effective (use of low cost natural substrates and only two enzymes), and the reaction could be performed in a one-pot system.

TL;DR: Trypsin-PCL fibrous membranes may be useful for concomitant proteolytic and separation commercial applications and retained catalytic activity and the observed reusability can be explained by a heterogeneous distribution of the enzyme within the polymer fiber influenced by the electrostatic field during the electrospinning process.

TL;DR: From these findings, ALA biosynthesis from glucose was successfully demonstrated and this study is the first to report ALA overproduction in C. glutamicum via metabolic engineering.

TL;DR: It is shown that three ligands that are closely associated with the T1 copper in SLAC play a key role in maintaining enzymatic activity and its suitability as a template for engineering with the aim of widening its potential range of industrial applications.

TL;DR: In patients older than 50 years with moderate or severe chronic knee pain, neither laser nor needle acupuncture conferred benefit over sham for pain or function, and the findings do not support acupuncture for these patients.

TL;DR: In this study, a novel glucose biosensor was fabricated by reconstitutional immobilization of glucose oxidase (GOx) onto a poly(glycidyl methacrylate-co-vinylferrocene) (poly(GMA- co-VFc)) film coated pencil graphite electrode (PGE).

TL;DR: The engineered novel xylanase exhibited superior enzymatic performance and showed promise as an excellent candidate for industrial application due to its high specific activity, stability and soluble protein yield.

TL;DR: This study reports a method for continuous hydrolysis of carboxymethyl cellulose (CMC) by using cross-linked cellulase aggregate (XCA) trapped inside a membrane, which is very stable under continuously flowing solutions.

TL;DR: Molecular modeling and dynamics simulations unveil the high conformational stability of the overall scaffold of CGL with high flexibility at the non-structural regions.