Journal ArticleDOI
Structure, function and regulation of the DNA-binding protein Dps and its role in acid and oxidative stress resistance in Escherichia coli: a review.
L.N. Calhoun,Y.M. Kwon +1 more
Reads0
Chats0
TLDR
It is proposed that Dps itself plays a role in gene regulation during starvation, ultimately making the cell more resistant to cytotoxic assaults by controlling the expression of genes necessary for (or deleterious to) stress resistance.Abstract:
Dps, the DNA-binding protein from starved cells, is capable of providing protection to cells during exposure to severe environmental assaults; including oxidative stress and nutritional deprivation. The structure and function of Dps have been the subject of numerous studies and have been examined in several bacteria that possess Dps or a structural/functional homologue of the protein. Additionally, the involvement of Dps in stress resistance has been researched extensively as well. The ability of Dps to provide multifaceted protection is based on three intrinsic properties of the protein: DNA binding, iron sequestration, and its ferroxidase activity. These properties also make Dps extremely important in iron and hydrogen peroxide detoxification and acid resistance as well. Regulation of Dps expression in E. coli is complex and partially dependent on the physiological state of the cell. Furthermore, it is proposed that Dps itself plays a role in gene regulation during starvation, ultimately making the cell more resistant to cytotoxic assaults by controlling the expression of genes necessary for (or deleterious to) stress resistance. The current review focuses on the aforementioned properties of Dps in E. coli, its prototypic organism. The consequences of elucidating the protective mechanisms of this protein are far-reaching, as Dps homologues have been identified in over 1000 distantly related bacteria and Archaea. Moreover, the prevalence of Dps and Dps-like proteins in bacteria suggests that protection involving DNA and iron sequestration is crucial and widespread in prokaryotes.read more
Citations
More filters
Journal ArticleDOI
Ferritin: a versatile building block for bionanotechnology.
TL;DR: This paper presents a meta-analyses of the chiral stationary phase of the response of the immune system to various types of materials and shows clear patterns of decline in the immune systems of mice treated with these materials.
Book ChapterDOI
Shiga toxin-producing Escherichia coli.
TL;DR: Since the non-O157 STEC are responsible for a large portion of STEC-related illnesses, more extensive studies on their physiology, genetics, pathogenicity, and evolution are needed in order to develop more effective control strategies.
Journal ArticleDOI
Mechanisms of Acid Resistance in Escherichia coli
Usheer Kanjee,Walid A. Houry +1 more
TL;DR: The enterobacterium Escherichia coli uses a range of physiological, metabolic, and proton-consuming acid resistance mechanisms in order to survive acid stresses as low as pH 2.0.
Journal ArticleDOI
Microbial response to acid stress: mechanisms and applications.
Ningzi Guan,Long Liu +1 more
TL;DR: This review summarizes acid stress response mechanisms of microbial cells, illustrates the application of microbial acid tolerance in industry, and prospect the introduction of systems and synthetic biology to further explore the acid tolerance mechanisms and construct a microbial cell factory for valuable chemicals.
References
More filters
Book
DNA Repair and Mutagenesis
TL;DR: Nucleotide excision repair in mammalian cells: genes and proteins Mismatch repair The SOS response and recombinational repair in prokaryotes Mutagenesis in proKaryote Mutagenisation in eukaryotes Other DNA damage tolerance responses in eUKaryotes.
Journal ArticleDOI
Bacterial iron homeostasis
TL;DR: The expression of the iron homeostatic machinery is subject to iron-dependent global control ensuring that iron acquisition, storage and consumption are geared to iron availability and that intracellular levels of free iron do not reach toxic levels.
Journal ArticleDOI
Rate of depurination of native deoxyribonucleic acid.
Tomas Lindahl,Barbro Nyberg +1 more
Journal ArticleDOI
Oxidative stress responses in Escherichia coli and Salmonella typhimurium.
TL;DR: The products of Oxy-R- and SoxRS-regulated genes, such as catalases and superoxide dismutases, are involved in the prevention of oxidative damage, whereas others play a role in the repair of oxidativeDamage.
Journal ArticleDOI
The N-end rule: functions, mysteries, uses
TL;DR: The N-end rule relates the in vivo half-life of a protein to the identity of its N-terminal residue and is discussed, and its applications are considered.