Open AccessJournal Article
Studies of Prostaglandins in Rat Mammary Tumors Induced by 7,12-Dimethylbenz(a)anthracene
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Thin-layer chromatography and use of labeled substrates showed that prostaglandins were also synthesized in mammary gland tumors, and the synthetic activity appeared to be greater in the aberrant tissue than in the tissue of normal mammary glands.Abstract:
Studies are reported on the detection and synthesis of prostaglandins in the tissues of normal mammary glands and in mammary tumors induced by i.v. injection of 7,12-dimethylbenz(a)anthracene into female Sprague-Dawley rats. Thin-layer chromatography, using silicic acid and argentation adsorption techniques, indicated that prostaglandin E2 was the major prostaglandin of both tumorous and normal mammary gland tissue. The prostaglandin E concentration, determined by radioimmunoassay, was greater in the tissues of the mammary tumors than in the mammary glands of the normal control animals. Use of labeled substrates showed that prostaglandins were also synthesized in mammary gland tumors, and the synthetic activity appeared to be greater in the aberrant tissue than in the tissue of normal mammary glands.read more
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References
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Journal ArticleDOI
The release of prostaglandins from lung and other tissues
Priscilla J. Piper,J. Vane +1 more
Journal ArticleDOI
Radioimmunoassay measurement of prostaglandins E, A, and F in human plasma.
TL;DR: The details of a radioimmunoassay capable of measuring as 5 pg of prostaglandin A, E, and F (PGA, PGE, and PGF) in human and rat plasma are described and appears to be of considerable experimental as well as clinical interest.
Journal ArticleDOI
Prostaglandins and related factors: xix. thin-layer chromatography of prostaglandins.
Krister Gréen,Bengt Samuelsson +1 more
TL;DR: In this paper, the authors described methods for separation of all known prostaglandins and some derivatives as free acids and methyl esters by thin-layer chromatography both on analytical and preparative scales.
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Regulation of Cell Motility by Cyclic AMP
TL;DR: At present, no strong evidence connects contact inhibition of movement and growth, but as transformation often disturbs both properties a connexion between them may exist.
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