Journal ArticleDOI
Studies on the role of factor Ts in polypeptide synthesis.
TLDR
The possibility is discussed that Ts is required to dissociate the Tu-GDP complex formed during the binding of AA-tRNA to ribosomes.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1970-03-01. It has received 108 citations till now. The article focuses on the topics: GTP'.read more
Citations
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Journal ArticleDOI
Purification and properties of rabbit reticulocyte elongation factor
TL;DR: Elongation factor 1 appears to show very little, if any, turnover in aminoacyl-tRNA binding, but in the presence of elongation factor 2, under conditions where polymerization occurs, elongationfactor 1 functions catalytically.
Book ChapterDOI
Qbeta RNA replicase and protein synthesis elongation factors EF-Tu and EF-Ts.
Journal ArticleDOI
Multiple forms of elongation factor 1 from calf brain
TL;DR: Heavy and light forms of elongation factor 1 from calf brain have been partially purified and it is found that EF-1(L) reacts with GTP and aminoacyl-tRNA more efficiently than EF-2(H), which contains significant amounts of free and esterified cholesterol.
Journal ArticleDOI
Magnetic resonance studies of the manganese guanosine di- and triphosphate complexes with elongation factor Tu.
G E Wilson,M Cohn +1 more
TL;DR: The frequency dependence of the PRR indicates that the electron spin relaxation time, T1e, is the dominant process modulating the Mn(II)-H2O interaction of the EF-Tu-MnGDP complex and consequently determines the correlation time.
Journal ArticleDOI
Protein synthetic ability of Escherichia coli valine transfer RNA with pseudouridine, ribothymidine, and other uridine-derived residues replaced by 5-fluorouridine.
TL;DR: There is no essential requirement for pseudouridine or ribothymidine in the GTψC loop of tRNA for its proper functioning in protein synthesis in vitro, confirming earlier work that dihydrouridine and 4-thiouridine are also not essential.
References
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Journal ArticleDOI
A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter
TL;DR: A modification of the naphthalene-dioxane-PPO liquid scintillator has been described which will allow up to 3.0 ml of an aqueous solution to be counted as mentioned in this paper.
Journal ArticleDOI
Hydrolysis of Guanosine 5'-Triphosphate Associated with Binding of Aminoacyl Transfer Ribonucleic Acid to Ribosomes
TL;DR: It is concluded that, although GTP hydrolysis resulted from the binding of aminoacyl-tRNA to ribosomes in a 1:1 stoichiometry, the two reactions were not tightly coupled under all experimental conditions.
Journal ArticleDOI
Peptide Chain Elongation: GTP Cleavage catalysed by Factors binding Aminoacyl-Transfer RNA to the Ribosome
TL;DR: At least two molecules of GTP may be hydrolysed during the addition of an amino-acid to a growing peptide chain.
Journal ArticleDOI
Evidence for a guanine nucleotide-aminoacyl-RNA complex as an intermediate in the enzymatic transfer of aminoacyl-RNA to ribosomes
TL;DR: Preliminary data indicate that F-I catalyzes the formation of a guanine nucleotide-aminoacyl-RNA complex which may be the intermediate product formed in the “enzymatic” transfer of aminoacyL-RNA to ribosomes.
Journal ArticleDOI
Formation and properties of the aminoacyl transfer ribonucleic acid-guanosine triphosphate-protein complex.
TL;DR: Evidence is provided that the aminoacyl-tRNA-GTP-FIu complex is an intermediate in the GTP-dependent binding of aminoacy-tRNAs to ribosomes, which is inhibited by chlortetracycline but not by deacylated tRNA.