Substrate Specificity of Soybean Lipoxidase
TLDR
Purified soybean lipoxidase was used to test the substrate specificity of all cis,cis-methylene-interrupted isomers of linoleic acid and the natural 9,12-isomer was found to be the best substrate, and the 13,16-isomers 50% as effective, the presence of calcium ions broadened the pattern of specificity.About:
This article is published in Journal of Biological Chemistry.The article was published on 1969-03-10 and is currently open access. It has received 101 citations till now. The article focuses on the topics: Degree of unsaturation & Double bond.read more
Citations
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Journal ArticleDOI
Arachidonate lipoxygenase in blood platelets
TL;DR: Blood platelets contain a lipoxygenase which converts eicosapolyenoic acids with at least two cis double bonds at the 8- and 11-position into l -12-hydroperoxy acids, and the enzyme responsible is found in the supernatant of broken platelets.
Journal ArticleDOI
Biohydrogenation of Unsaturated Fatty Acids VI. SOURCE OF HYDROGEN AND STEREOSPECIFICITY OF REDUCTION
I.S. Rosenfeld,S.B. Tove +1 more
TL;DR: The distribution of deuterium at the reduced carbon atoms shows an isotope effect and leads to the speculation that reduction occurs by addition of a proton and hydride ion mediated by an unknown carrier.
Journal ArticleDOI
Steric analysis of hydroperoxides formed by lipoxygenase oxygenation of linoleic acid.
TL;DR: Gas-liquid chromatography allowed separation of the diastereoisomeric derivatives and thus provided a sensitive means for determination of the absolute configuration of the parent unsaturated hydroxy ester.
Journal ArticleDOI
Oxygenation of unsaturated fatty acids by soybean lipoxygenase.
TL;DR: It is confirmed that oxygenation of fatty acids by lipoxygenase occurs with a kinetic lag period which can be abolished by adding product hydroperoxides and it is shown that the lag period may be extended as a result of inhibition of the enzyme by polyenoic acid substrates.
References
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Journal ArticleDOI
On the Specificity of the Oxygenation of Unsaturated Fatty Acids Catalyzed by Soybean Lipoxidase
Mats Hamberg,Bengt Samuelsson +1 more
TL;DR: The conversion of [13l-3H, 3-14C]8,11,14-eicosatrienoic acid is accompanied by an isotope effect, suggesting that the hydrogen removal occurs as the initial step of the reaction, and only the hydrogen of the l configuration is removed by the enzyme during the conversion.
Journal ArticleDOI
Linoleic acid and trilinolein as substrates for soybean lipoxidase (s).
TL;DR: Evidence has been presented that each of the fractions contains a different type of fat-peroxidizing enzyme, and experimental results indicate that lipoxidases may occur as two groups, i.e., those active on triglycerides and thoseactive on fatty acids.
Journal ArticleDOI
Effects of ethylenic bond position upon acyltransferase activity with isomeric cis,cis-octadecadienoyl coenzyme A thiol esters.
TL;DR: Coenzyme A thiol esters of the complete series of positional isomers of methylene-interrupted cis,cis-octadecadienoic acid were synthesized, indicating that the structure near carbon atoms 8, 9, and 10 is critical in the metabolism of polyunsaturated acids.
Related Papers (5)
On the Specificity of the Oxygenation of Unsaturated Fatty Acids Catalyzed by Soybean Lipoxidase
Mats Hamberg,Bengt Samuelsson +1 more