Open AccessJournal Article
Suppression of 7,12-Dimethylbenz(a)anthracene-induced Mammary Carcinogenesis in Rats by Resveratrol Role of Nuclear Factor-κB, Cyclooxygenase 2, and Matrix Metalloprotease 9
TLDR
Results suggest that resveratrol suppresses DMBA-induced mammary carcinogenesis, which correlates with down-regulation of NF-kappaB, cyclooxygenase-2, and matrix metalloprotease-9 expression.Abstract:
We have reported recently that resveratrol ( trans -3,4′,5-trihydroxystilbene), a polyphenolic phytoalexin found in grapes, fruits, and root extracts of the weed Polygonum cuspidatum , is a potent inhibitor of nuclear factor (NF)-κB activation. Because NF-κB suppression has been linked with chemoprevention, this prompted us to investigate the chemopreventive potential of resveratrol by testing it against mammary carcinogenesis induced by 7,12-dimethylbenz( a )anthracene (DMBA) in female Sprague Dawley rats. Dietary administration of resveratrol (10 ppm) had no effect on body weight gain and tumor volume but produced striking reductions in the incidence (45%; P P in situ (7 of 7), whereas treatment with resveratrol suppressed DMBA-induced ductal carcinoma. Immunohistochemistry and Western blot analysis revealed that resveratrol suppressed the DMBA-induced cyclooxygenase-2 and matrix metalloprotease-9 expression in the breast tumor. Gel shift analysis showed suppression of DMBA-induced NF-κB activation by resveratrol. Treatment of human breast cancer MCF-7 cells with resveratrol also suppressed the NF-κB activation and inhibited proliferation at S-G 2 -M phase. Overall, our results suggest that resveratrol suppresses DMBA-induced mammary carcinogenesis, which correlates with down-regulation of NF-κB, cyclooxygenase-2, and matrix metalloprotease-9 expression.read more
Citations
More filters
Book ChapterDOI
Chemopreventive Activity of Mediterranean Medicinal Plants
TL;DR: Generally, the use of plants, herbs or other natural products in medicines is since humans inhabited earth when humans were trying to find out which plants might be useful to fight several pains and aches, fever, dyspepsia, or wounds, and through the ages, humans learned which plants would cure different illnesses.
Journal ArticleDOI
Recent Overview of Resveratrol’s Beneficial Effects and Its Nano-Delivery Systems
Raghvendra A. Bohara,Nazish Tabassum,Mohan Singh,Giuseppe Gigli,Andrea Ragusa,Stefano Leporatti +5 more
TL;DR: The article summarizes the different potential applications of resveratrol as, for example, a therapeutic and disease-preventing anticancer and antiviral agent.
Journal ArticleDOI
Epigenetic Regulation and Dietary Control of Triple Negative Breast Cancer
TL;DR: The epigenetic mechanisms of regulation involved in cell pathways disrupted in TNBC are reviewed, with particular emphasis on dietary food components that may be exploited for the development of effective strategies for management of TNBC.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Cancer Chemopreventive Activity of Resveratrol, a Natural Product Derived from Grapes
Mei-Shiang Jang,Lining Cai,George Udeani,Karla Slowing,Cathy F. Thomas,Chris Beecher,Harry H. S. Fong,Norman R. Farnsworth,A. Douglas Kinghorn,Rajendra G. Mehta,Richard C. Moon,John M. Pezzuto +11 more
TL;DR: It is suggested that resveratrol, a common constituent of the human diet, merits investigation as a potential cancer chemopreventive agent in humans.
Journal ArticleDOI
Critique of Report on "Food, Nutrition and the Prevention of Cancer: A Global Perspective"
Journal ArticleDOI