Open AccessJournal Article
Suppression of 7,12-Dimethylbenz(a)anthracene-induced Mammary Carcinogenesis in Rats by Resveratrol Role of Nuclear Factor-κB, Cyclooxygenase 2, and Matrix Metalloprotease 9
TLDR
Results suggest that resveratrol suppresses DMBA-induced mammary carcinogenesis, which correlates with down-regulation of NF-kappaB, cyclooxygenase-2, and matrix metalloprotease-9 expression.Abstract:
We have reported recently that resveratrol ( trans -3,4′,5-trihydroxystilbene), a polyphenolic phytoalexin found in grapes, fruits, and root extracts of the weed Polygonum cuspidatum , is a potent inhibitor of nuclear factor (NF)-κB activation. Because NF-κB suppression has been linked with chemoprevention, this prompted us to investigate the chemopreventive potential of resveratrol by testing it against mammary carcinogenesis induced by 7,12-dimethylbenz( a )anthracene (DMBA) in female Sprague Dawley rats. Dietary administration of resveratrol (10 ppm) had no effect on body weight gain and tumor volume but produced striking reductions in the incidence (45%; P P in situ (7 of 7), whereas treatment with resveratrol suppressed DMBA-induced ductal carcinoma. Immunohistochemistry and Western blot analysis revealed that resveratrol suppressed the DMBA-induced cyclooxygenase-2 and matrix metalloprotease-9 expression in the breast tumor. Gel shift analysis showed suppression of DMBA-induced NF-κB activation by resveratrol. Treatment of human breast cancer MCF-7 cells with resveratrol also suppressed the NF-κB activation and inhibited proliferation at S-G 2 -M phase. Overall, our results suggest that resveratrol suppresses DMBA-induced mammary carcinogenesis, which correlates with down-regulation of NF-κB, cyclooxygenase-2, and matrix metalloprotease-9 expression.read more
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Resveratrol protects quail hepatocytes against heat stress: modulation of the Nrf2 transcription factor and heat shock proteins
TL;DR: The results of the present study suggest that supplemental resveratrol reduces oxidative stress in heat-stressed quails through modulating the hepatic heat shock proteins and nuclear transcription factors.
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Targeting of Aryl Hydrocarbon Receptor-Mediated Activation of Cyclooxygenase-2 Expression by the Indole-3-Carbinol Metabolite 3,3′-Diindolylmethane in Breast Cancer Cells
TL;DR: Data suggest that naturally occurring modulators of the AhR such as DIM may be effective agents for dietary strategies against epigenetic activation of COX-2 expression by AhR agonists.
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The combination of rapamycin and resveratrol blocks autophagy and induces apoptosis in breast cancer cells.
TL;DR: The results show that combination of these drugs maintains inhibition of mTORC1 signaling, while preventing upregulation of Akt activation and autophagy, causing apoptosis, and this combination was effective in estrogen receptor positive and negative breast cancer cells, underscoring its versatility.
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Effect of cis-resveratrol on genes involved in nuclear factor kappa B signaling.
TL;DR: In this paper, the effects of RESV (c-RESV), a polyphenol present in red wine, on an array of genes whose expression is controlled by nuclear factor kappa B (NF-nB) and whose transcriptional activation is critical in a number of pathologies (including some cardiovascular diseases).
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Resveratrol and apoptosis
TL;DR: It is demonstrated the existence of a plasma membrane receptor for resveratrol near the arginine–glycine–aspartate (RGD) recognition site on integrin αvβ3 that is involved in stilbene‐induced apoptosis of cancer cells.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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Cancer Chemopreventive Activity of Resveratrol, a Natural Product Derived from Grapes
Mei-Shiang Jang,Lining Cai,George Udeani,Karla Slowing,Cathy F. Thomas,Chris Beecher,Harry H. S. Fong,Norman R. Farnsworth,A. Douglas Kinghorn,Rajendra G. Mehta,Richard C. Moon,John M. Pezzuto +11 more
TL;DR: It is suggested that resveratrol, a common constituent of the human diet, merits investigation as a potential cancer chemopreventive agent in humans.
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Critique of Report on "Food, Nutrition and the Prevention of Cancer: A Global Perspective"
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