Open AccessJournal Article
Suppression of 7,12-Dimethylbenz(a)anthracene-induced Mammary Carcinogenesis in Rats by Resveratrol Role of Nuclear Factor-κB, Cyclooxygenase 2, and Matrix Metalloprotease 9
TLDR
Results suggest that resveratrol suppresses DMBA-induced mammary carcinogenesis, which correlates with down-regulation of NF-kappaB, cyclooxygenase-2, and matrix metalloprotease-9 expression.Abstract:
We have reported recently that resveratrol ( trans -3,4′,5-trihydroxystilbene), a polyphenolic phytoalexin found in grapes, fruits, and root extracts of the weed Polygonum cuspidatum , is a potent inhibitor of nuclear factor (NF)-κB activation. Because NF-κB suppression has been linked with chemoprevention, this prompted us to investigate the chemopreventive potential of resveratrol by testing it against mammary carcinogenesis induced by 7,12-dimethylbenz( a )anthracene (DMBA) in female Sprague Dawley rats. Dietary administration of resveratrol (10 ppm) had no effect on body weight gain and tumor volume but produced striking reductions in the incidence (45%; P P in situ (7 of 7), whereas treatment with resveratrol suppressed DMBA-induced ductal carcinoma. Immunohistochemistry and Western blot analysis revealed that resveratrol suppressed the DMBA-induced cyclooxygenase-2 and matrix metalloprotease-9 expression in the breast tumor. Gel shift analysis showed suppression of DMBA-induced NF-κB activation by resveratrol. Treatment of human breast cancer MCF-7 cells with resveratrol also suppressed the NF-κB activation and inhibited proliferation at S-G 2 -M phase. Overall, our results suggest that resveratrol suppresses DMBA-induced mammary carcinogenesis, which correlates with down-regulation of NF-κB, cyclooxygenase-2, and matrix metalloprotease-9 expression.read more
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The role of phytochemicals in inhibition of cancer and inflammation: New directions and perspectives
TL;DR: In this paper, a review summarizes some of the molecular mechanisms through which several phytochemicals may inhibit inflammation and cancer and discusses basic definitions, ethical and clinical aspects and some guidelines for the use of phytochemical agents as chemopreventive or therapeutic agents.
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Breaking the relay in deregulated cellular signal transduction as a rationale for chemoprevention with anti-inflammatory phytochemicals
TL;DR: Modulation of cellular signaling involved in chronic inflammatory response by anti-inflammatory phytochemicals may comprise a rational and pragmatic strategy in molecular target-based chemoprevention.
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Resveratrol modulates the phosphoinositide 3-kinase pathway through an estrogen receptor α-dependent mechanism: Relevance in cell proliferation
TL;DR: A mechanism through which RES could inhibit survival and proliferation of estrogen‐responsive cells by interfering with an ERα‐associated PI3K pathway is proposed, following a process that could be independent of the nuclear functions of the ERα.
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Resveratrol: An overview of its anti-cancer mechanisms.
TL;DR: The role of resveratrol in the prevention and therapy of cancer with particular focus on colorectal and skin cancer is highlighted and the molecular mechanisms underlying its chemopreventive and therapeutic activity are discussed.
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Mitochondria, calcium, and calpain are key mediators of resveratrol-induced apoptosis in breast cancer.
TL;DR: A critical role for mitochondria is suggested not only in the intrinsic apoptotic pathway but also in the Ca2+ and calpain-dependent cell death initiated by RES, which may prove useful as a nontoxic alternative for breast cancer treatment.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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Cancer Chemopreventive Activity of Resveratrol, a Natural Product Derived from Grapes
Mei-Shiang Jang,Lining Cai,George Udeani,Karla Slowing,Cathy F. Thomas,Chris Beecher,Harry H. S. Fong,Norman R. Farnsworth,A. Douglas Kinghorn,Rajendra G. Mehta,Richard C. Moon,John M. Pezzuto +11 more
TL;DR: It is suggested that resveratrol, a common constituent of the human diet, merits investigation as a potential cancer chemopreventive agent in humans.
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Critique of Report on "Food, Nutrition and the Prevention of Cancer: A Global Perspective"
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