Journal ArticleDOI
Synchronised transmembrane insertion and glycosylation of a nascent membrane protein
TLDR
A surprisingly precise sequence by which the nascent chain of this membrane glycoprotein is glycosylated in two steps is revealed, which has important implications for the mechanisms of membrane assembly.Abstract:
Studies of the synthesis and incorporation of the vesicular stomatitis virus glycoprotein into membranes in a synchronised cell-free system demonstrate a tight coupling between polypeptide synthesis and membrane insertion, as a result of which the nascent chain crosses the membrane. The studies reveal a surprisingly precise sequence by which the nascent chain of this membrane glycoprotein is glycosylated in two steps. These findings have important implications for the mechanisms of membrane assembly.read more
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Inhibitors of the Biosynthesis and Processing of N-Linked Oligosaccharide Chains
TL;DR: A number of inhibitors have been identified that interfere with glycoprotein biosynthesis, processing, or transport, such as tunicamycin, tridecaptin, and flavomycin this paper.
Inhibitors of the biosynthesis and processing of n-linked
TL;DR: A number of glycoproteins have oligosaccharides linked to protein in a GlcNAc----asparagine bond that are either of the complex, the high-mannose or the hybrid structure and a number of inhibitors have been identified that interfere with glycoprotein biosynthesis, processing, or transport.
Journal ArticleDOI
Structure of the influenza virus glycoprotein antigen neuraminidase at 2.9 Å resolution
TL;DR: The tetrameric enzyme has circular 4-fold symmetry stabilized in part by metal ions bound on the symmetry axis, and sugar residues are attached to four of the five potential glycosylation sequences, and in one case contribute to the interaction between subunits in the tetramer.
Journal ArticleDOI
The spontaneous insertion of proteins into and across membranes: the helical hairpin hypothesis
TL;DR: It is proposed that the initial event in the secretion of proteins across membranes and their insertion into membranes is the spontaneous penetration of the hydrophobic portion of the bilayer by a helical hairpin.
Journal ArticleDOI
Mechanisms for the incorporation of proteins in membranes and organelles.
TL;DR: Mechanisms for the transfer of newly synthesized polypeptides to their sites of function in different subcellular membranes and organelles are considered, and models in which specific features of the polypePTides serve as signals to direct them along selected sub cellular pathways to their final destination are discussed.
References
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Journal ArticleDOI
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
TL;DR: It is demonstrated that pre-exposure of the film to a brief flash of light greatly increases the sensitivity of fluorography, because it corrects the non-linear relationship between radioactivity of the sample and absorbance of theFilm image.
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Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
TL;DR: In this article, it was shown that in vitro completion of these nascent light chains resulted in the synthesis of some chains having the same mol wt as the authentic secreted light chain, because of completion of in vivo proteolytically processed chains and of other chains which, due to the completion of unprocessed chains, have the same moll wt, as the precursor of the light chain.
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TRANSFER OF PROTEINS ACROSS MEMBRANES II. Reconstitution of Functional Rough Microsomes from Heterologous Components
TL;DR: These results establish unequivocally that the information for segregation of a translation product is encoded in the mRNA itself, not in the protein- synthesizing apparatus; this provides strong evidence in support of the signal hypothesis.
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Membrane Asymmetry: The nature of membrane asymmetry provides clues to the puzzle of how membranes are assembled.
James E. Rothman,John Lenard +1 more
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Membrane Structure: Some General Principles
TL;DR: The arrangement of lipids and some proteins in the erythrocyte membrane has been discussed and the conclusions are listed here as a set of general guidelines for the structure of membranes of higher organisms: some of these rules may be wrong.