Taxonomy of the canine Mollicutes by 16S rRNA gene and 16S/23S rRNA intergenic spacer region sequence comparison.
Victoria J. Chalker,Joe Brownlie +1 more
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TLDR
The taxonomy of canine Mollicutes is described, based on phylogenetic analysis of 16S rRNA gene and 16S/23S r RNA intergenic spacer (IGS) region sequences, and the two untyped Mycoplasma strains, HRC 689 and VJC 358, were found to be distinct from all known canine mycoplasmas and all published myCoplasma 16S sRNA gene sequences.Abstract:
The taxonomy of canine Mollicutes is described, based on phylogenetic analysis of 16S rRNA gene and 16S/23S rRNA intergenic spacer (IGS) region sequences. The nucleotide sequences of the 16S rRNA gene of two untyped mycoplasmas and the IGS region of 11 Mycoplasma species were determined and used for phylogenetic analysis. The two untyped Mycoplasma strains, HRC 689 and VJC 358, were found to be distinct from all known canine mycoplasmas and all published mycoplasma 16S rRNA gene sequences.read more
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Journal ArticleDOI
16S rDNA PCR and denaturing gradient gel electrophoresis; a single generic test for detecting and differentiating Mycoplasma species.
TL;DR: The development of a new diagnostic test based on PCR of the 16S rRNA gene with Mycoplasma-specific primers and separation of the PCR product according to primary sequence using denaturing gradient gel electrophoresis (DGGE).
Journal ArticleDOI
Revised minimal standards for description of new species of the class Mollicutes (division Tenericutes)
TL;DR: Revised standards for novel species of the class Mollicutes are proposed that reflect recent advances in molecular systematics and the species concept for prokaryotes.
Journal ArticleDOI
Mycoplasmas associated with canine infectious respiratory disease
Victoria J. Chalker,Wanda M. A. Owen,Caren J.I Paterson,Emily N. Barker,Harriet W. Brooks,Andrew N. Rycroft,Joe Brownlie +6 more
TL;DR: The species of mycoplasma present throughout the respiratory tract of dogs with and without CIRD were determined and isolation of M. cynos was correlated with an increased severity of CIRD, younger age and a longer time in the kennel.
Journal ArticleDOI
RNA polymerase beta subunit (rpoB) gene and the 16S-23S rRNA intergenic transcribed spacer region (ITS) as complementary molecular markers in addition to the 16S rRNA gene for phylogenetic analysis and identification of the species of the family Mycoplasmataceae.
TL;DR: Three-target sequence analysis was demonstrated to be a reliable and useful taxonomic tool for the species differentiation within the family Mycoplasmataceae based on their phylogenetic relatedness and pairwise sequence similarities.
Journal ArticleDOI
Genetic analysis of housekeeping genes of members of the genus Acholeplasma: phylogeny and complementary molecular markers to the 16S rRNA gene.
Dmitriy V. Volokhov,Alexander A. Neverov,Joseph George,Hyesuk Kong,Sue X. Liu,Christine Anderson,Maureen K. Davidson,Vladimir Chizhikov +7 more
TL;DR: The multilocus phylogenetic analysis of Acholeplasma multilocale sequence data strongly indicated that this organism is most closely related to the genera Mesoplasma and Entomoplasma (family entomoplasmataceae) and form the branch with mesoplasma seiffertii, Mesopl plasma syrphidae, and Mesoplasia photuris.
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Book ChapterDOI
D1 – Introductory remarks
TL;DR: This chapter reviews the current status and evaluation of the newer diagnostic methods for the measurement of a specific antibody in the infection of bovine mycoplasmas.