Taxonomy of the canine Mollicutes by 16S rRNA gene and 16S/23S rRNA intergenic spacer region sequence comparison.
Victoria J. Chalker,Joe Brownlie +1 more
Reads0
Chats0
TLDR
The taxonomy of canine Mollicutes is described, based on phylogenetic analysis of 16S rRNA gene and 16S/23S r RNA intergenic spacer (IGS) region sequences, and the two untyped Mycoplasma strains, HRC 689 and VJC 358, were found to be distinct from all known canine mycoplasmas and all published myCoplasma 16S sRNA gene sequences.Abstract:
The taxonomy of canine Mollicutes is described, based on phylogenetic analysis of 16S rRNA gene and 16S/23S rRNA intergenic spacer (IGS) region sequences. The nucleotide sequences of the 16S rRNA gene of two untyped mycoplasmas and the IGS region of 11 Mycoplasma species were determined and used for phylogenetic analysis. The two untyped Mycoplasma strains, HRC 689 and VJC 358, were found to be distinct from all known canine mycoplasmas and all published mycoplasma 16S rRNA gene sequences.read more
Citations
More filters
Journal ArticleDOI
Age-related presence of selected viral and bacterial pathogens in paraffin-embedded lung samples of dogs with pneumonia
TL;DR: This retrospective approach yielded valuable data on the presence, absence and prevalence of certain respiratory pathogens in dogs with pneumonia on the basis of archived formalin-fixed and paraffin wax-embedded lung samples.
Journal ArticleDOI
Characterization of a novel Mycoplasma cynos real-time PCR assay:
Rebecca L. Tallmadge,Renee R. Anderson,Patrick K. Mitchell,Zachary C Forbes,Brenda G. Werner,Gloria Gioia,Paolo Moroni,Amy L. Glaser,Anil J. Thachil,Laura B. Goodman +9 more
TL;DR: A new open-source real-time PCR (rtPCR) assay for M. cynos that performs well under standard rtPCR conditions and has high specificity, as confirmed by sequence confirmation on ISR PCR amplicons obtained from clinical specimens.
Journal ArticleDOI
Molecular detection and seroprevalence of mycoplasmas in clinicaly healthy working dogs
Sara Suhadolc Scholten,Natasa Tozon,Saša Koprivec,Kaja Felda,Mateja Florjančič,Dušan Benčina,Brigita Slavec +6 more
TL;DR: In this study seroprevalence and prevalence of mycoplasmas in clinically healthy dogs were studied and dot-immunobinding assay (DIBA) was used as a serological test for the detection of specific antibodies to Mycoplasma cynos, MyCoplasma canis and My coplasma molare and consensus PCR for detection of genes for 16S rRNA or 16S/23S IGS region of my cplasmas was used.
Journal ArticleDOI
The detection and differentiation of canine respiratory pathogens using oligonucleotide microarrays.
TL;DR: The oligonucleotide microarray provides a highly efficient diagnosis alternative that could be applied to clinical usage, greatly assisting in disease therapy and control.
Journal ArticleDOI
The influence of Mycoplasma species on human and canine semen quality: a review
TL;DR: This review summarizes the current state of knowledge about the impact of Mycoplasma on canine and human male fertility as well as the prospects of further development in this field.
References
More filters
Journal ArticleDOI
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
TL;DR: ClUSTAL X is a new windows interface for the widely-used progressive multiple sequence alignment program CLUSTAL W, providing an integrated system for performing multiple sequence and profile alignments and analysing the results.
Book ChapterDOI
Rapid and Sensitive Sequence Comparison with FASTP and FASTA.
TL;DR: FASTA and FASTP were designed to identify protein sequences that have descended from a common ancestor and they have proved very useful for this task, but it is not clear that NWS-based programs would be more successful in finding distantly related members of the G-protein-coupled receptor family.
Journal ArticleDOI
Likelihood-mapping: A simple method to visualize phylogenetic content of a sequence alignment
TL;DR: A graphical method, likelihood-mapping, is introduced, to visualize the phylogenetic content of a set of aligned sequences, based on an analysis of the maximum likelihoods for the three fully resolved tree topologies that can be computed for four sequences.