Journal ArticleDOI
Temperature-sensitive mammalian cell line blocked in mitosis.
TLDR
A hamster cell mutant is isolated in which mitosis is blocked in metaphase and the processing of ribosomal RNA10 is inhibited.Abstract:
SEVERAL temperature-sensitive mammalian cell mutants in culture have been isolated1–10, including three defective in protein syntheses6, cytokinesis5 and the processing of ribosomal RNA10, respectively. I have now isolated a hamster cell mutant in which mitosis is blocked in metaphase.read more
Citations
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On the nature of hereditable variation in cultured somatic cells.
Journal ArticleDOI
Genes controlling essential cell-cycle functions in Drosophila melanogaster.
Maurizio Gatti,Bruce S. Baker +1 more
TL;DR: The terminal phenotypes exhibited by these Drosophila mitotic mutants are remarkably similar to those observed in mammalian cell-cycle mutants, suggesting that these diverse organisms use a common genetic logic to regulate and integrate the events of the cell cycle.
Journal ArticleDOI
Cell division from a genetic perspective
TL;DR: A novel view of the eukaryotic cell cycle is taking form as genetic strategies borrowed from investigations of microbial gene regulation and bacteriophage morphogenesis are being applied to the process of cell division.
Journal ArticleDOI
Injection of anticentromere antibodies in interphase disrupts events required for chromosome movement at mitosis.
TL;DR: The results suggest that the CENP antigens are involved in two essential interphase events that are required for centromere action in mitosis and the structural maturation of the kinetochore that begins at prophase.
Journal ArticleDOI
Activated N-ras controls the transformed phenotype of HT1080 human fibrosarcoma cells
Hugh Paterson,Brian Reeves,Robin Brown,Alan Hall,Mark E. Furth,Johannes L. Bos,Philip H. Jones,Christopher J. Marshall +7 more
TL;DR: Analysis of sporadic tumors derived from the revertant cells reveals an increased dosage of the transforming allele and results imply direct participation of an N-ras oncogene in maintaining the transformed phenotype of a human tumor cell line.
References
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Journal ArticleDOI
Synthesis of RNA and protein during mitosis in mammalian tissue culture cells.
D.M. Prescott,M.A. Bender +1 more
TL;DR: The capacity of these cells to incorporate 3 H histidine appeared to decline as the duration of metaphase arrest increased, and the transfer of RNA from nucleus to cytoplasm was faster for the more rapidly growing hamster cells than for HeLa.
Journal Article
Studies on unbalanced growth in tissue culture. I. Induction and consequences of thymidine deficiency.
TL;DR: Thymidine-deficient growth of HeLa cells induced by either of two agents, amethopterin or 5-fluorouracil-2′-deoxyriboside, resulted in an unbalanced growth syndrome strikingly similar to that described for bacteria.
Journal ArticleDOI
Temperature sensitive mutants of BHK 21 cells.
TL;DR: Temperature sensitive mutant cells were isolated from animal cells by multiple culturing in the presence of 5-fluoro-2′-deoxyuridine and properties of the mutants are described.
Journal ArticleDOI
Isolation of temperature-sensitive mutants of L-cells.
TL;DR: Procedures are described for the isolation of conditional lethal mutants of mouse L-60T cells that were temperature sensitive by the following criteria: colony-forming ability, growth in suspension culture, and rate of uptake of tritiated-thymidine.
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