Journal ArticleDOI
Tenascin: cDNA cloning and induction by TGF-beta.
TLDR
The accumulation of the two extracellular matrix proteins in the medium was differentially affected by fetal calf serum and TGF‐beta and the increase was in both cases higher for tenascin, and the induction ofTenascin was compared with fibronectin.Abstract:
cDNA clones coding for tenascin, an extracellular matrix glycoprotein with a restricted tissue distribution, were isolated from a chicken fibroblast cDNA expression library using a specific tenascin antiserum. Antibodies eluted from the cDNA-encoded fusion proteins reacted exclusively with tenascin. Limited trypsin treatment of purified tenascin resulted in a peptide which confirmed the deduced protein sequences. The largest clone encoding 632 amino acids showed a cysteine-rich region containing 13 consecutive epidermal growth factor-like repeats of unusual uniformity. Northern blot analysis revealed 8- to 9-kb messages. Tenascin is shown to be induced in vitro by fetal calf serum as well as by transforming growth factor beta (TGF-beta). A 4-fold increase in tenascin secretion by chick embryo fibroblasts was seen after TGF-beta treatment. The induction of tenascin protein synthesis was preceded by an increase of tenascin mRNA as determined by Northern blot analysis. The induction of tenascin was compared with fibronectin. The accumulation of the two extracellular matrix proteins in the medium was differentially affected by fetal calf serum and TGF-beta and the increase was in both cases higher for tenascin.read more
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Transforming growth factor-beta in disease: the dark side of tissue repair.
Wayne A. Border,Erkki Ruoslahti +1 more
Journal ArticleDOI
The cellular and molecular basis of peripheral nerve regeneration.
Susan Y. Fu,Tessa Gordon +1 more
TL;DR: Axonal regeneration may be facilitated by new strategies that enhance the growth potential of neurons and optimize the growth support of the distal nerve stump in combination with prompt nerve repair.
Journal ArticleDOI
Transforming Growth Factor-β
TL;DR: Transforming growth factor-β (TGF-β) as mentioned in this paper is the cytokine with the broadest range of activities in repair of injured tissue, based both on the variety of cell types that produce and/or respond to it and on the spectrum of its cellular responses.
Journal ArticleDOI
Natural inhibitor of transforming growth factor-|[beta]| protects against scarring in experimental kidney disease
Wayne A. Border,Nancy A. Noble,Tatsuo Yamamoto,John R. Harper,Yu Yamaguchi,Michael D. Pierschbacher,Erkki Ruoslahti +6 more
TL;DR: It is reported here that administration of decorin inhibits the increased production of extracellular matrix and attenuates manifestations of disease, confirming the hypothesis that decorin may eventually prove to be clinically useful in diseases associated with overproduction of TGF-β.
Journal ArticleDOI
Interaction of the small interstitial proteoglycans biglycan, decorin and fibromodulin with transforming growth factor beta.
A Hildebrand,M Romarís,Lars Melholt Rasmussen,Dick Heinegård,D R Twardzik,D R Twardzik,Wayne A. Border,E Ruoslahti,E Ruoslahti +8 more
TL;DR: In this article, the interactions of three proteoglycans of the decorin family, decorin, biglycan and fibromodulin, with transforming growth factor beta (TGF-beta) were analyzed.