Open AccessJournal Article
The acetylcholinesterase gene Ace: a diagnostic marker for the Pipiens and Quinquefasciatus forms of the Culex pipiens complex.
Denis Bourguet,Dina M. Fonseca,G Vourch,Marie-Pierre Dubois,Fabrice Chandre,Carlo Severini,Michel Raymond +6 more
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TLDR
In this article, a method based on a restriction enzyme polymorphism in polymerase chain reaction-amplified DNA was described, in which the presence or absence of one restriction site discriminates Culex pipiens, C. quinquefasciatus and their hybrids.Abstract:
The taxonomy of the Culex pipiens complex remains a controversial issue in mosquito systematics. Based on morphologic characters, 2 allopatric taxa are recognized, namely Cx. pipiens (including the form "molestus") in temperate areas and Cx. quinquefasciatus in tropical areas. Here we report on variability at the nucleotide level of an acetylcholinesterase gene in several strains and natural populations of this species complex. Few polymorphisms were found in coding regions within a subspecies but many polymorphisms were observed between subspecies in noncoding regions. We describe a method based on a restriction enzyme polymorphism in polymerase chain reaction-amplified DNA, in which the presence or absence of one restriction site discriminates Cx. pipiens, Cx. quinquefasciatus, and their hybrids. This technique reliably discriminates mosquitoes from more than 30 worldwide strains or populations. Polymerase chain reaction amplification of specific alleles may also be a useful tool for characterizing specific alleles of each sibling taxon.read more
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Journal ArticleDOI
Rapid assays for identification of members of the culex (culex) pipiens complex, their hybrids, and other sibling species (diptera: culicidae)
Julie L. Smith,Dina M. Fonseca +1 more
TL;DR: This work introduces and describes a series of PCR-based assays that use polymorphisms in the second intron of the acetylcholinesterase-2 (ace-2) locus for the identification of members of the Cx.
Biochemical genetics and genomics of insect esterases
TL;DR: This article reviews the biochemistry and genetics of insect esterases, focusing mainly on developments, from a genomics perspective and the bulk of it is organized around the major clades of insectEsterases as revealed by the comparative genomic analysis.
Book ChapterDOI
Biochemical Genetics and Genomics of Insect Esterases
TL;DR: The Comprehensive Molecular Insect Science (CMIIS) as discussed by the authors is a complete reference encompassing important developments and achievements in modern insect science, including reviews on the ecdysone receptor, lipocalins, and bacterial toxins.
Journal ArticleDOI
Host-feeding habits of Culex and other mosquitoes (Diptera: Culicidae) in the Borough of Queens in New York City, with characters and techniques for identification of Culex mosquitoes.
Charles S. Apperson,Bruce A. Harrison,Thomas R. Unnasch,Hassan K. Hassan,William S. Irby,Harry M. Savage,Stephen E. Aspen,D. Wesley Watson,Leopoldo M. Rueda,Barry Engber,Roger S. Nasci +10 more
TL;DR: Host-feeding patterns of mosquitoes collected in the Borough of Queens in New York City in July and August 2000 were investigated using an indirect ELISA and a polymerase chain reaction (PCR)-heteroduplex assay, and species identifications of Culex mosquitoes made using morphological characteristics were confirmed with a PCR assay that employed species-specific primers.
Journal ArticleDOI
Independent Duplications of the Acetylcholinesterase Gene Conferring Insecticide Resistance in the Mosquito Culex pipiens
Pierrick Labbé,Arnaud Berthomieu,Claire Berticat,Haoues Alout,Michel Raymond,Thomas Lenormand,Mylène Weill +6 more
TL;DR: Molecular evidence is provided for the occurrence of several (at least 3) independent duplications of the ace-1 locus in the mosquito Culex pipiens, selected in response to insecticide pressure that probably occurred very recently (<40 years ago).
References
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Wolbachia endosymbionts responsible for various alterations of sexuality in arthropods.
TL;DR: Three molecular lineages can be identified on the basis of 16S as well as 23S sequences, and Wolbachia spread by horizontal transfer across host lineages as evidenced by their diversification which occurred long after that of their hosts, and by the non-congruence of the phylogenetic relationships of symbionts and their hosts.
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Worldwide migration of amplified insecticide resistance genes in mosquitoes.
TL;DR: The results suggest that amplified esterase B2 genes originated from an initial event that has subsequently spread organophosphate insecticide resistance by migration, and that electrophoretically dissimilar esterases B varied considerably.
Journal Article
PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single-base changes.
TL;DR: The results indicate that with proper optimization, all alleles were reliably distinguished and PCR amplification of specific alleles to detect 69 polymorphic or mutant alleles was reliably distinguished.
Journal Article
Carbamate-resistance in mosquitos. Selection of Culex pipiens fatigans Wiedemann (=C. quinquefasciatus Say) for resistance to Baygon.
TL;DR: The authors consider the relatively slow rate of development of resistance to Baygon, the reduced expressivity of the resistance character in adults, and its specificity for the selective agent and for closely related carbamates to be encouraging indications as to the usefulness of this class of compounds for mosquito control.
Journal ArticleDOI
Phylogeny of fourteen Culex mosquito species, including the Culex pipiens complex, inferred from the internal transcribed spacers of ribosomal DNA.
TL;DR: Phylogenetic analysis of ITS‐1 and ITS‐2 sequences from members of the Cx.
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Rapid assays for identification of members of the culex (culex) pipiens complex, their hybrids, and other sibling species (diptera: culicidae)
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