Journal ArticleDOI
The Application of Stereological Methods for Estimating Structural Parameters in the Human Heart
Yong Tang,Yong Tang,Jens R. Nyengaard,Johnnie B. Andersen,Ulrik Baandrup,Hans Jørgen G. Gundersen +5 more
Reads0
Chats0
TLDR
In the six hearts constituting this study, the anticipated correlation between left ventricular volume and global estimates such as total number of capillaries and cardiomyocytes was observed and the applied principles of unbiased stereology were applied.Abstract:
This study describes and exemplifies generally applicable design-based stereological methods for obtaining quantitative estimates of the numbers and sizes of capillaries, cardiomyocytes, and cardiomyocyte nuclei in immersion-fixed human left ventricles (N = 6). The design-based stereological methods are valid in all cardiac investigations onto quantifying changes in structure and function as seen under various conditions such as during development, aging, hypertrophy, and following ischemia/reperfusion. The applied principles of unbiased stereology were as follows: 1) uniform random sampling was taken at all levels, also in respect to orientations, for estimates of length and mean sizes. 2) All global structural quantities were estimated as total quantity = density x volume of the left ventricle. As an example, the left ventricle contains 1.5 x 10(9) capillaries with a total length of just below 200 km. 3) Stereological methods were used for estimating the volume density, surface area density, and length density of capillaries and cardiomyocytes. The numerical density of cardiomyocyte nuclei and capillaries was estimated, using the optical and physical disector, respectively. 4) In all local quantities, "size" was estimated either directly, using unbiased estimators to obtain the average individual size and size distribution parameters, or indirectly, using the relationship that: average size = total quantity/total number. In the six hearts constituting this study, we observed the anticipated correlation between left ventricular volume and global estimates such as total number of capillaries. There were no correlation between local quantities and total left ventricular volume (e.g., average star volume of individual cardiomyocytes).read more
Citations
More filters
Journal ArticleDOI
Cardiomyocyte proliferation contributes to heart growth in young humans.
Mariya Mollova,Kevin R. Bersell,Stuart Walsh,Jainy Savla,Lala Tanmoy Das,Shin-Young Park,Leslie E. Silberstein,Leslie E. Silberstein,Cristobal G. dos Remedios,Dionne A. Graham,Steven D. Colan,Bernhard Kühn +11 more
TL;DR: Examining the cellular growth mechanisms of the left ventricle on a set of healthy hearts from humans aged 0–59 y shows that cardiomyocyte proliferation contributes to developmental heart growth in young humans, suggesting that children and adolescents may be able to regenerate myocardium, and that abnormal cardiomeocyte proliferation may be involved in myocardial diseases that affect this population.
Journal ArticleDOI
Recounting Cardiac Cellular Composition
Pingzhu Zhou,William T. Pu +1 more
TL;DR: The question of the cellular composition of the modern heart is revisited using modern techniques and reagents and a useful new cell surface marker for cardiac fibroblasts is validated, which may prove broadly useful to cardiac biologists.
Journal ArticleDOI
Cardiac regeneration based on mechanisms of cardiomyocyte proliferation and differentiation
TL;DR: This work will discuss approaches for examining cardiomyocyte regeneration, review the specific advantages, challenges, and controversies, and recommend approaches for interpretation of results.
Journal ArticleDOI
Quantitative microscopy of the lung - a problem-based approach Part 2: Stereological parameters and study designs in various diseases of the respiratory tract
Christian Mühlfeld,Matthias Ochs +1 more
TL;DR: The present pathophysiological knowledge about acute lung injury, diffuse parenchymal lung diseases, emphysema, pulmonary hypertension, and asthma is analyzed to come up with recommendations for the disease-specific application of stereological principles for obtaining relevant parameters.
Journal ArticleDOI
Telocytes and putative stem cells in ageing human heart.
Laurentiu M. Popescu,Antoanela Curici,Enshi Wang,Hao Zhang,Shengshou Hu,Mihaela Gherghiceanu +5 more
TL;DR: It is found that interstitial area gradually increases with age from 31.3 ± 4.9% in newborns to 41 ± 5.2% in adults, and cardiac fibroblasts are the most prevalent cell type in the heart and account for about 20% of myocardial volume.
References
More filters
Journal ArticleDOI
The efficiency of systematic sampling in stereology and its prediction
TL;DR: A set of very simple estimators of efficiency are presented and illustrated with a variety of biological examples and a nomogram for predicting the necessary number of points when performing point counting is provided.
Journal ArticleDOI
Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator
TL;DR: A stereological method for obtaining estimates of the total number of neurons in five major subdivisions of the rat hippocampus is described, and a number of new stereological methods that have particular relevance to the quantitative study of the structure of the nervous system are briefly described in an appendix.
Journal ArticleDOI
Some new, simple and efficient stereological methods and their use in pathological research and diagnosis.
Hj. Gundersen,Tf. Bendtsen,Lise Korbo,Niels Marcussen,Arne Møller,K. Nielsen,Jr. Nyengaard,Bente Pakkenberg,Flemming Brandt Sørensen,A. Vesterby +9 more
TL;DR: Methods for estimating the volume, surface area and length of any structure are described in this review and the principles on which stereology is based and the necessary sampling procedures are described and illustrated with examples.
Journal ArticleDOI
The unbiased estimation of number and sizes of arbitrary particles using the disector
TL;DR: A three‐dimensional counting rule and its integral test system, the disector, for obtaining unbiased estimates of the number of arbitrary particles in a specimen is presented.
Journal ArticleDOI
Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson
TL;DR: The full range of estimators is described, some of them for the first time, some in an improved form, several in more than one version, and all of them under the single, absolute requirement that one can in fact identify what one is quantifying on sections.
Related Papers (5)
Dynamics of Cell Generation and Turnover in the Human Heart
Olaf Bergmann,Sofia Zdunek,Anastasia Felker,Mehran Salehpour,Kanar Alkass,Samuel Bernard,Staffan L. Sjostrom,Mirosława Szewczykowska,Teresa Jackowska,Cris dos Remedios,Torsten Malm,Michaela Andrä,Ramadan Jashari,Jens R. Nyengaard,Göran Possnert,Stefan Jovinge,Stefan Jovinge,Stefan Jovinge,Henrik Druid,Jonas Frisén +19 more