Matthias Ochs

TL;DR: The lung poses special challenges, some of which are outlined below and discussed in later sections: Heterogeneity of lung structure requires standardized preparation methods, and the practice of picking specific samples or sections often fails to account for regional heterogeneity, leading to biased conclusions with respect to the whole organ.

TL;DR: A design-based stereologic approach was used for the direct and unbiased estimation of alveolar number in the human lung, based on two-dimensional topology in three-dimensional space and free of assumptions on the shape, size, or spatial orientation ofAlveoli.

TL;DR: PSC-derived AEC2s provide a platform for disease modeling and future functional regeneration of the distal lung, and exhibit self-renewal capacity, and display additional A EC2 functional capacities.

TL;DR: The pitfalls of lung morphometry are discussed and solutions, from specimen preparation to the sampling scheme in multiple stages, are presented for obtaining unbiased estimates of morphometric parameters such as volumes, surfaces, lengths, and numbers.

TL;DR: Although L(m) is not a robust parameter of internal lung structure because it crucially depends on lung volume, it is still a valid measure for which accurate and efficient methods are available that yield additional parameters such as size distribution or alveolar surface area.