Journal ArticleDOI
The cauliflower mosaic virus 35s promoter : combinatorial regulation of transcription in plants
Philip N. Benfey,Nam-Hai Chua +1 more
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TLDR
The expression patterns conferred by specific combinations of 35S subdomains differ in tobacco and petunia, indicating that a combinatorial code of cisregulatory elements may be interpreted differently in different species.Abstract:
Appropriate regulation of transcription in higher plants requires specific cis elements in the regulatory regions of genes and their corresponding trans-acting proteins. Analysis of the cauliflower mosaic virus (CaMV) 35S promoter has contributed to the understanding of transcriptional regulatory mechanisms. The intact 35S promoter confers constitutive expression upon heterologous genes in most plants. Dissection into subdomains that are able to confer tissue-specific gene expression has demonstrated that the promoter has a modular organization. When selected subdomains are combined, they confer expression not detected from the isolated subdomains, suggesting that synergistic interactions occur among cis elements. The expression patterns conferred by specific combinations of 35S subdomains differ in tobacco and petunia. This indicates that a combinatorial code of cisregulatory elements may be interpreted differently in different species.read more
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Photosynthesis under stressful environments: An overview
Muhammad Ashraf,Philip Harris +1 more
TL;DR: Progress made during the last two decades in producing transgenic lines of different C3 crops with enhanced photosynthetic performance is discussed, which was reached by either the overexpression of C3 enzymes or transcription factors or the incorporation of genes encoding C4 enzymes into C3 plants.
Journal ArticleDOI
Arabidopsis LEAFY COTYLEDON1 Is Sufficient to Induce Embryo Development in Vegetative Cells
Tamar Lotan,Masa-aki Ohto,Kelly Matsudaira Yee,Marilyn A. L. West,Russell S. Lo,Raymond W. Kwong,Kazutoshi Yamagishi,Robert L. Fischer,Robert B. Goldberg,John J. Harada +9 more
TL;DR: The results suggest that LEC1 is an important regulator of embryo development that activates the transcription of genes required for both embryo morphogenesis and cellular differentiation.
Journal ArticleDOI
SHATTERPROOF MADS-box genes control seed dispersal in Arabidopsis
TL;DR: It is shown that the closely related SHATTERPROOF (SHP1) and SHP2 ) MADS-box genes are required for fruit dehiscence in Arabidopsis, and that further analysis of the molecular events underlying fruit deHiscence may allow genetic manipulation of pod shatter in crop plants.
Patent
Promoter (FLt) for the full-length transcript of peanut chlorotic streak caulimovirus (PCLSV) and expression of chimeric genes in plants
Indu B. Maiti,Robert J. Shepperd +1 more
TL;DR: In this paper, the isolation, modification and use of wild-type and modified viral FLt promoters of PClSV in the expression of chimeric genes in plant cells is described.
Journal ArticleDOI
A knotted1-like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants.
TL;DR: The hypothesis that class 1 kn1-like genes play a role in morphogenesis is supported by the characterization of two genes, KNAT1 and KNAT2 that were cloned from Arabidopsis using the kn1 homeobox as a heterologous probe.
References
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Journal ArticleDOI
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
TL;DR: GUS is very stable, and tissue extracts continue to show high levels of GUS activity after prolonged storage, and Histochemical analysis has been used to demonstrate the localization of gene activity in cells and tissues of transformed plants.
Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
Journal ArticleDOI
Steroid Receptor Regulated Transcription of Specific Genes and Gene Networks
TL;DR: Information is provided on how to identify the Steroid Receptors, Receptor Binding Sites, and other mechanisms that aid in the identification of the receptors and their locations in the genome.
Journal ArticleDOI
Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter
TL;DR: The effects of 5′ deletions in a plant viral promoter in tobacco callus as well as in regenerated plants, includ ing different plant tissues, are analysed to allow a more direct assessment of deletion effects.
Journal ArticleDOI
Duplication of CaMV 35S promoter sequences creates a strong enhancer for plant genes
TL;DR: A variant of the cauliflower mosaic virus 35S promoter with transcriptional activity approximately tenfold higher than that of the natural promoter was constructed by tandem duplication of 250 base pairs of upstream sequences, which should be very useful for obtaining high levels of expression of foreign genes in transgenic plants.