The culture of plant cells with ammonium salts as the sole nitrogen source.
O.L. Gamborg,J.P. Shyluk +1 more
TLDR
Soybean cell suspension cultures grew on defined media with ammonium as the sole nitrogen source if Krebs cycle acids were added, and ammonium enhanced the activity of glutamate dehydrogenase.Abstract:
Soybean cell suspension cultures grew on defined media with ammonium as the sole nitrogen source if Krebs cycle acids were added. Satisfactory growth was obtained with ammonium salts of citrate, malate, fumarate, or succinate, when compared with the regular medium containing nitrate and ammonium. Little or no growth occurred when ammonium salts of shikimate, tartrate, acetate, carbonate, or sulfate were used. The cells also grew well with l-glutamine as nitrogen source. The specific activities of glutamine synthetase and isocitrate dehydrogenase (nicotinamide adenine dinucleotide phosphate) were lower than in cells grown on a nitrate medium, but ammonium enhanced the activity of glutamate dehydrogenase. Cells of soybean, wheat, and flax have been cultured for an extended period on the ammonium citrate medium.read more
Citations
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Journal ArticleDOI
Ammonium and nitrate nutrition of plants
R. J. Haynes,K. M. Goh +1 more
TL;DR: In this article, the effects of ammonium plus nitrate as a nitrogen source were investigated and they showed that ammonium toxicity is associated with changes in metabolic changes characteristic of toxicity.
Journal ArticleDOI
Plant tissue culture media.
TL;DR: The development of the various types of tissue culture has been based on empirical approaches, and some of the observations recorded in the literature may not be typical for plant cells.
Journal ArticleDOI
Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase
Andreas J. Müller,Reinhard Grafe +1 more
TL;DR: Differences between the NR-negative mutants with respect to chlorate resistance suggest that chlorate inhibits cultured N tabacum cells not only via its NR-catalysed conversion to chlorite, but also by NR-independent mechanisms.
Journal ArticleDOI
Growth and ion uptake by wheat supplied nitrogen as nitrate, or ammonium, or both
W. J. Cox,H. M. Reisenauer +1 more
TL;DR: The effects of concentration and source (NH4, NO3, and NO3 plus NH4) of added N on the rate of growth, final yield, and content and rate of intake of N, P, K, Ca, Mg and S by wheat seedlings were evaluated as mentioned in this paper.
Journal ArticleDOI
Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon
TL;DR: Shikonin derivatives were produced from cell suspension cultures of Lithospermum erythrorhizon for the first time as mentioned in this paper, and the results of studies on various culture media showed that the type of nitrogen source in the medium greatly influenced the production of these derivatives.
References
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Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Journal ArticleDOI
Regulation of glutamine synthetase. I. Purification and properties of glutamine synthetase from Escherichia coli.
TL;DR: Analytical ultracentrifugation, amino acid analyses, manganese determination, and light-scattering measurements have been performed, and indicate that the E. coli enzyme exhibits marked specificity for the l isomer of glutamate.
Journal ArticleDOI
The effects of amino acids and ammonium on the growth of plant cells in suspension culture.
TL;DR: Addition of citrate (5 mM) relieved the inhibitory effects of ammonium in soybean and wheat cells but not in the Haplopappus cells, and higher cell yields were obtained when ammonium (2 mM) or glutamine also was present.
Journal ArticleDOI
Culture methods and detection of glucanases in suspension cultures of wheat and barley.
Oluf L. Gamborg,D. E. Eveleigh +1 more
TL;DR: The presence of a laminaranase (endo-β-(1 → 3)-D-glucan glucanohydrolase (EC 3.2.1.99)) that did not attack lichenan was established and the culture media of the wheat contained an oligosaccharide which on acid hydrolysis yielded galactose, arabinose, and xylose.
Journal ArticleDOI
Regulation of nitrate reductase in cultured tobacco cells
TL;DR: The nitrate reductase of tobacco cells grown in suspension culture on a chemically defined medium is induced by nitrate, and the enzyme decays in cells grown into nitrate starvation, and it is not formed in cells growing on casein hydrolysate as the sole nitrogen source.
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