The Cyclase-associated protein Cap1 is important for proper regulation of infection-related morphogenesis in Magnaporthe oryzae.
Reads0
Chats0
TLDR
The results indicated that CAP1 is important for the activation of adenylate cyclase, appressorium morphogenesis, and plant infection in M. oryzae and may also play a role in feedback inhibition of Ras2 signaling when Pmk1 is activated.Abstract:
Surface recognition and penetration are critical steps in the infection cycle of many plant pathogenic fungi. In Magnaporthe oryzae, cAMP signaling is involved in surface recognition and pathogenesis. Deletion of the MAC1 adenylate cyclase gene affected appressorium formation and plant infection. In this study, we used the affinity purification approach to identify proteins that are associated with Mac1 in vivo. One of the Mac1-interacting proteins is the adenylate cyclase-associated protein named Cap1. CAP genes are well-conserved in phytopathogenic fungi but none of them have been functionally characterized. Deletion of CAP1 blocked the effects of a dominant RAS2 allele and resulted in defects in invasive growth and a reduced intracellular cAMP level. The Δcap1 mutant was defective in germ tube growth, appressorium formation, and formation of typical blast lesions. Cap1-GFP had an actin-like localization pattern, localizing to the apical regions in vegetative hyphae, at the periphery of developing appressoria, and in circular structures at the base of mature appressoria. Interestingly, Cap1, similar to LifeAct, did not localize to the apical regions in invasive hyphae, suggesting that the apical actin cytoskeleton differs between vegetative and invasive hyphae. Domain deletion analysis indicated that the proline-rich region P2 but not the actin-binding domain (AB) of Cap1 was responsible for its subcellular localization. Nevertheless, the AB domain of Cap1 must be important for its function because CAP1ΔAB only partially rescued the Δcap1 mutant. Furthermore, exogenous cAMP induced the formation of appressorium-like structures in non-germinated conidia in CAP1ΔAB transformants. This novel observation suggested that AB domain deletion may result in overstimulation of appressorium formation by cAMP treatment. Overall, our results indicated that CAP1 is important for the activation of adenylate cyclase, appressorium morphogenesis, and plant infection in M. oryzae. CAP1 may also play a role in feedback inhibition of Ras2 signaling when Pmk1 is activated.read more
Citations
More filters
Journal ArticleDOI
Regulation of appressorium development in pathogenic fungi
TL;DR: Appressorium development is linked to cell cycle checkpoints controlling morphogenesis andSeptin-mediated actin re-modelling is essential for appressorium function, and focal secretion of effectors occurs duringappressorium infection.
Journal ArticleDOI
Protein Kinases in Plant-Pathogenic Fungi: Conserved Regulators of Infection
TL;DR: Understanding how conserved PK signaling networks have been recruited during the evolution of fungal pathogenicity not only advances knowledge of the highly elaborate infection process but may also lead to the development of novel strategies for the control of plant disease.
Journal ArticleDOI
Genetic control of infection-related development in Magnaporthe oryzae.
TL;DR: This review focuses on the key signaling pathways and recent advances in Magnaporthe oryzae, which is a model for studying fungal-plant interactions and a number of upstream genes and downstream targets of the cAMP-PKA and mitogen-activated protein (MAP) pathways have been identified.
Journal ArticleDOI
The FgHOG1 pathway regulates hyphal growth, stress responses, and plant infection in Fusarium graminearum.
Dawei Zheng,Shijie Zhang,Xiaoying Zhou,Chenfang Wang,Ping Xiang,Qian Zheng,Jin-Rong Xu,Jin-Rong Xu +7 more
TL;DR: The results indicate that the FgSsk2-FgPbs2- FgHog1 MAPK cascade is important for regulating hyphal growth, branching, plant infection, and hyperosmotic and general stress responses in F. graminearum.
Journal ArticleDOI
Molecular tools for functional genomics in filamentous fungi: recent advances and new strategies.
TL;DR: This review presents an up-to-date review on the different molecular tools and latest strategies that have been successfully used in functional genomics in filamentous fungi, including methods for genetic transformation, construction of random mutant libraries, and the analysis of gene function.
References
More filters
Journal ArticleDOI
Lifeact: a versatile marker to visualize F-actin
Julia Riedl,Alvaro H. Crevenna,Kai Kessenbrock,Jerry Haochen Yu,Dorothee Neukirchen,Michal Bista,Frank Bradke,Dieter E. Jenne,Tad A. Holak,Zena Werb,Michael Sixt,Roland Wedlich-Söldner +11 more
TL;DR: Lifeact, a 17-amino-acid peptide, is described, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.
Journal ArticleDOI
MAP kinase and cAMP signaling regulate infection structure formation and pathogenic growth in the rice blast fungus Magnaporthe grisea.
Jin-Rong Xu,John E. Hamer +1 more
TL;DR: It is demonstrated that PMK1 is part of a highly conserved MAP kinase signal transduction pathway that acts cooperatively with a cAMP signaling pathway for fungal pathogenesis.
Journal ArticleDOI
Roles for Rice Membrane Dynamics and Plasmodesmata during Biotrophic Invasion by the Blast Fungus
TL;DR: Analysis of biotrophic blast invasion will significantly contribute to the understanding of normal plant processes and allow the characterization of secreted fungal effectors that affect these processes.
Journal ArticleDOI
Autophagic Fungal Cell Death Is Necessary for Infection by the Rice Blast Fungus
TL;DR: The formation of an appressorium required, sequentially, the completion of mitosis, nuclear migration, and death of the conidium (fungal spore) from which the infection originated.
Related Papers (5)
MAP kinase and cAMP signaling regulate infection structure formation and pathogenic growth in the rice blast fungus Magnaporthe grisea.
Jin-Rong Xu,John E. Hamer +1 more