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The determination of total lipides in blood serum

Warren M. Sperry, +1 more
- 01 Mar 1955 - 
- Vol. 213, Iss: 1, pp 69-76
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This article is published in Journal of Biological Chemistry.The article was published on 1955-03-01 and is currently open access. It has received 227 citations till now. The article focuses on the topics: Blood serum.

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Citations
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Journal ArticleDOI

A simple method for the isolation and purification of total lipides from animal tissues.

TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI

The metabolism of low density lipoprotein in familial type II hyperlipoproteinemia

TL;DR: The pathologic elevation of plasma LDL concentration in the individuals with type II hyperlipoproteinemia studied here is due to a decreased fractional rate of LDL degradation rather than to an abnormality of LDL synthesis, which may be secondary to an underlying abnormality in lipid metabolism.
Journal ArticleDOI

The quantitative estimation of cerebrosides in nervous tissue.

TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
Journal ArticleDOI

Serum Lipids in Infection

TL;DR: Lipoprotein electrophoresis demonstrated that the hyperlipidemia in patients with severe infection caused by gram-negative bacilli was related to increased levels of pre-beta-lipoproteins, which resulted from increased concentrations of serum triglycerides and free cholesterol.
Journal ArticleDOI

Effect of very low-density lipoproteins on lipid transfer in incubated serum.

TL;DR: In this article, it was shown that reciprocal transfer of cholesterol esters for glycerides in human serum lipoproteins can occur, and the HDL class was then the principal contributor of the cholesterol ester.
References
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Journal ArticleDOI

Preparation of lipide extracts from brain tissue.

TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
Journal ArticleDOI

Colorimetric determination of blood lipides.

TL;DR: The determinations reported here followed a conventional alcohol-ether extraction and subsequent reextraction in petroleum ether, and it has been found that urea, even in amounts considerably in excess of what might occur, produces no color change in the dichromate reagent.
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