The distribution of dust mite allergen in the houses of patients with asthma.
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The results suggested that natural exposure to this dust allergen allows occasional fecal particles to enter the lungs and that these particles contain very concentrated allerGEN.Abstract:
Using an inhibition radioimmunoassay for the major allergen from Dermatophagoides pteronyssinus (antigen P1), we studied the distribution of this dust allergen in the houses of patients with asthma. Both bed and floor dust samples contained a wide range of antigen P1, 100 to 100,000 ng/g of fine dust, and this concentration correlated well with the number of mite bodies (r = 0.81, p less than 0.001). We were unable to detect antigen P1 in the air of undisturbed rooms. However, during domestic activity, between 1 and 30 ng were collected on a filter than sampled air for 45 min at 17 L/min. Using a cascade impactor it was shown that greater than 80% of the airborne antigen P1 was associated with particles greater than 10 mu in diameter. Some of the particles containing allergen could be identified because they formed precipitin rings when impacted onto agarose containing rabbit antimite antiserum. These particles had the physical appearance of mite feces, which are the major source of antigen P1 in mite cultures. The results suggested that natural exposure to this dust allergen allows occasional fecal particles to enter the lungs and that these particles contain very concentrated allergen.read more
Citations
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Design and Methods of the National Cooperative Inner-City Asthma Study
Herman Mitchell,Yvonne D. Senturia,Peter J. Gergen,Dean Baker,Dean Baker,Christine L.M. Joseph,Kathleen Mcniff-Mortimer,H. James Wedner,Ellen F. Crain,Peyton A. Eggleston,Richard Evans,Meyer Kattan,Carolyn M. Kercsmar,Frederick E. Leickly,Floyd J. Malveaux,Ernestine Smartt,Kevin B. Weiss +16 more
TL;DR: The design and methods of the broad‐based initial Phase I epidemiologic investigation of the National Cooperative Inner‐City Asthma Study, established to identify and then intervene on those factors which are related to asthma morbidity among children in the inner‐city, are described.
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The role of water temperature and laundry procedures in reducing house dust mite populations and allergen content of bedding
Lindy G. McDonald,Euan R. Tovey +1 more
TL;DR: Dry cleaning did not reduce the allergen concentration of the dust, although most, if not all, mites were killed, and a cold cycle of laundry washing with or without laundry powder did not remove most live mites from bedding.
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Mite antigen in house dust: relationship with different housing characteristics in the Netherlands.
TL;DR: The Der p I concentrations in dust from carpeted bedroom floors and mattresses were positively associated with the average relative humidity in the bedroom over a period of 3–6 weeks in a subset of the homes where relative humidity was measured.
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Measurement of airborne mite antigen in homes of asthmatic children
TL;DR: Air sampling is a more appropriate method of assessing antigen exposure than dust sampling for asthmatic patients because at no level was the association as strong as that with air measurements.
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The Role of Dust Mites in Allergy.
TL;DR: Beyond their direct importance as a major allergen source, an understanding of dust mites leads to insights into the nature of atopy and of allergic sensitization in general.
References
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Journal Article
Deposition and retention models for internal dosimetry of the human respiratory tract. Task group on lung dynamics.
Journal ArticleDOI
Mite faeces are a major source of house dust allergens
TL;DR: Measurements from a radioimmunoassay show that more than 95% of the allergen accumulating in mite cultures is associated with faecal particles.
Journal ArticleDOI
The house-dust mite (Dermatophagoides pteronyssinus) and the allergens it produces. Identity with the house-dust allergen
TL;DR: The finding of the house-dust mite seems to supply an answer to all factors, and the keystone of the theory is formed by the fact that after being made equivalent, extracts of house dust and mite cultures gave skin reactions which were both qualitatively and quantitatively indistinguishable.
Journal ArticleDOI
Mites and house-dust allergy in bronchial asthma
TL;DR: The mites in a large number of dust samples from houses of patients with bronchial asthma were identified and counted: the most common species was Dermatophagoides pteronyssinus, and it was particularly abundant in mattress dust.
Journal ArticleDOI
Effects of anti-mite measures on children with mite-sensitive asthma: a controlled trial.
TL;DR: Mite counts and tests for mite antigen were performed on samples of dust taken from the bedding of 53 children with mite-sensitive asthma and no significant differences emerged in the progress of the two groups, both tending to improve.
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