Journal ArticleDOI
The Effect of Actinoquinol with Hyaluronic Acid in Eye Drops on the Optical Properties and Oxidative Damage of the Rabbit Cornea Irradiated with UVB Rays
TLDR
In conclusion, actinoquinol‐hyaluronic acid eye drops decreased changes in corneal optics and suppressed oxidative damage in the UVB‐irradiated cornea, however, the effectiveCorneal protection by these eye drops was limited to the lower UVB dose.Abstract:
Irradiation of the cornea with UVB rays leads to its oxidative damage, swelling and increased light absorption. We investigated changes in the corneal optics (evaluated by changes of corneal hydration and light absorption) and microscopical disturbances of corneas irradiated with UVB rays as influenced by eye drops containing actinoquinol with hyaluronic acid. Rabbit corneas were irradiated with a daily dose of 0.5 or 1.01 J cm )2 of UVB rays (312 nm) for 4 days. During irradiation, the eye drops were applied on the right eye and buffered saline (or hyaluronic acid) on the left eye. On day 5 the rabbits were sacrificed and the corneas examined spectrophotometrically for light absorption. The corneal thickness (hydration) was measured using a pachymeter. Corneas of some other rabbits were examined immunohistochemically. After buffered saline treatment UVB rays evoked changes in the corneal optics and induced oxidative damage of the corneas. After actinoquinol-hyaluronic acid application, these changes were diminished. Hyaluronic acid alone was less effective. In conclusion, actinoquinol-hyaluronic acid eye drops decreased changes in corneal optics and suppressed oxidative damage in the UVB-irradiated cornea. However, the effective corneal protection by these eye drops was limited to the lower UVB dose.read more
Citations
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Journal ArticleDOI
The role of oxidative stress in corneal diseases and injuries.
Jitka Cejkova,Cestmir Cejka +1 more
TL;DR: This review aims to summarize immunohistochemical changes in severe corneal injuries and diseases in which oxidative stress has been proved.
Journal ArticleDOI
Hyaluronic acid-dependent protection against UVB-damaged human corneal cells.
Ji-Min Li,Hsiu-Chuan Chou,Ssu-Han Wang,Chieh-Lin Wu,Yi-Wen Chen,Szu-Ting Lin,Yu-Hua Chen,Hong-Lin Chan +7 more
TL;DR: Proteomic analysis showed that HMW‐HA might modulate cytoskeleton regulation, signal transduction, biosynthesis, redox regulation, and protein folding to stimulate wound healing and to prevent these UVB‐damaged cells from cell death.
Journal ArticleDOI
Central corneal thickness considered an index of corneal hydration of the UVB irradiated rabbit cornea as influenced by UVB absorber.
TL;DR: It is suggested that actinoquinol/ hyaluronic acid drops might be helpful for the human eye in the defence against photooxidative and other oxidative processes.
Journal ArticleDOI
A New Nanosecond UV Laser at 355 nm: Early Results of Corneal Flap Cutting in a Rabbit Model
Andrea Trost,Clemens Strohmaier,Barbara Bogner,Christian Runge,Alexandra Kaser-Eichberger,Karolina Krefft,Alfred Vogel,Norbert Linz,Sebastian Freidank,Andrea Hilpert,Inge Zimmermann,Herbert A. Reitsamer,Herbert A. Reit +12 more
TL;DR: This new UV laser used for corneal flap cutting in an animal model revealed no epi- nor endothelial damage at energies feasible for cornea flap cutting, and is suitable for refractive surgery, awaiting its test in a chronic environment.
Journal ArticleDOI
Hydration and Transparency of the Rabbit Cornea Irradiated with UVB-Doses of 0.25 J/cm 2 and 0.5 J/cm 2 Compared with Equivalent UVB Radiation Exposure Reaching the Human Cornea from Sunlight
TL;DR: Although comparison of experimental and outdoor conditions are only approximate, the results in rabbits point to the danger for the human eye from UVB radiation when short stays in sunlight are repeated for several consecutive days without UV protection.
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