Journal ArticleDOI
The effect of platelet-derived growth factor on morphology and motility of human glial cells
K Mellstroöm,Anna-Stina Höglund,Monica Nistér,Carl-Henrik Heldin,Bengt Westermark,Uno Lindberg +5 more
Reads0
Chats0
TLDR
It is documented in this work that one of the earliest effects of PDGF on serum-starved glial cells is an induction of intensive motile activity.Abstract:
Platelet-derived growth factor (PDGF) is a mitogen for several cell types in culture. It is documented in this work that one of the earliest effects of PDGF on serum-starved glial cells is an induction of intensive motile activity. Within the first minute after the addition of PDGF thin membrane lamellae grow out around almost all of the cell circumference. Later, circular arrangements of small ruffles appear on the dorsal surface of the cells. These rings of ruffles vary in size and some encircle almost the whole cell. The organization of the peripheral weave of microfilaments in the PDGF-induced advancing lamellae was closely similar to that of normally growing cells. In the regions of the circular arrangements of ruffles there was an extensive reorganization of the surface actin with unusual arrangements of microfilament bundles and polygonal networks. There was also a general intensification of the translocation of membrane ruffles and spikes from the cell periphery towards the centre of the cell, increased micropinocytotic activity and shuttling of intracellular particles.read more
Citations
More filters
Journal ArticleDOI
The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors.
Anne J. Ridley,Alan Hall +1 more
TL;DR: Rho, a ras-related GTP-binding protein, rapidly stimulated stress fiber and focal adhesion formation when microinjected into serum-starved Swiss 3T3 cells, implying that rho is essential specifically for the coordinated assembly of focal adhesions and stress fibers induced by growth factors.
Journal ArticleDOI
Structure of the receptor for platelet-derived growth factor helps define a family of closely related growth factor receptors
Yosef Yarden,Jaime Escobedo,Wun-Jing Kuang,Teresa L. Yang-Feng,T. O. Daniel,P. M. Tremble,Ellson Y. Chen,M. E. Ando,Richard N. Harkins,Uta Francke,Victor A. Fried,Axel Ullrich,Lewis T. Williams +12 more
TL;DR: The primary structure of the receptor for platelet-derived growth factor (PDGF), determined by means of cloning a cDNA that encodes the murine pre-PDGF receptor, is closely related to that of the v-kit oncogene product and the receptors for macrophage colony stimulating factor (CSF-1).
Journal ArticleDOI
Growth factors: Mechanism of action and relation to oncogenes
Journal ArticleDOI
Specific interaction between phosphatidylinositol 4,5-bisphosphate and profilactin
Ingrid Lassing,Uno Lindberg +1 more
TL;DR: It is demonstrated here that isolated profilin, as well as the profilactin complex, interacts with anionic phospholipids, suggesting the possibility of a relationship between the induction of actin filament formation and the increased activity in the phosphatidylinositol cycle seen as a result of ligand–receptor interactions in various systems.
Journal ArticleDOI
Foot and mouth: podosomes, invadopodia and circular dorsal ruffles
TL;DR: The plasma membrane of many motile cells undergoes highly regulated protrusions and invaginations that support the formation of podosomes, invadopodia and circular dorsal ruffles, which function is to help the cell as it migrates, attaches and invades.
References
More filters
Journal ArticleDOI
The preparation of 131i-labelled human growth hormone of high specific radioactivity
TL;DR: The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated.
Journal ArticleDOI
Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
W M Hunter,F. C. Greenwood +1 more
TL;DR: Current procedures for the immunological assay of protein hormones in human plasma require the routine preparation of hormones labelled with iodine-131 of high specific activity, and this work demonstrates the importance of knowing the carrier and removal status of iodine.
Journal ArticleDOI
Transforming gene product of Rous sarcoma virus phosphorylates tyrosine
TL;DR: It is inferred that pp60src is a novel protein kinase and that the modification of proteins via the phosphorylation of tyrosine is essential to the malignant transformation of cells by Rous sarcoma virus.
Journal ArticleDOI
125I-labeled human epidermal growth factor. Binding, internalization, and degradation in human fibroblasts.
TL;DR: The data are consistent with a mechanism in which 125I-hEGF initially is bound to the cell surface and subsequently is internlized before degradation, and the binding capacity of these cells is restored by incubation in a serum-containing medium.
Journal ArticleDOI
Epidermal growth factor-receptor-protein kinase interactions. Co-purification of receptor and epidermal growth factor-enhanced phosphorylation activity.
TL;DR: Analysis of the affinity-purified preparation by sodium dodecyl sulfate-gel electrophoresis indicates the presence of one major protein band of molecular weight 150,000 which is the receptor for EGF and is a substrate of the phosphorylation reaction.
Related Papers (5)
Specific interaction between phosphatidylinositol 4,5-bisphosphate and profilactin
Ingrid Lassing,Uno Lindberg +1 more