Journal ArticleDOI
The evaluation of morphological characteristics of human spermatozoa according to stricter criteria
TLDR
It can be concluded that the method developed in the laboratory and which resulted in the use of stricter criteria for the evaluation of sperm morphology is a practical, reliable and repeatable method and has a good prognostic value for the prediction of expected IVF fertilization, the hamster test and hemizona assay.Abstract:
The evaluation of the morphology of human spermatozoa varies widely between and sometimes even within laboratories. The purpose of this study was to determine whether the method that has been developed in our laboratory and which resulted in the use of stricter criteria for the evaluation of sperm morphology is a practical, reliable and repeatable method and to establish the within and between observer variations. The criteria used for a 'normal' spermatozoon are based on the appearance of spermatozoa found in the mucus of the upper endocervical canal. The results of the morphological evaluations of 26 samples by four observers were statistically analysed by various methods. The method of Barnett showed a high degree of relative accuracy between observers with error variances of between 2.89 and 19.67 as well as high Spearman rank correlation coefficients of between 0.8675 and 0.6537 (P less than 0.0003). The Spearman correlation coefficient for 15 duplicate evaluations by one observer was 0.9650 (P less than 0.0001) while the coefficients of variation for repeated evaluations of single samples were also within acceptable limits. Based on these results, the method described in this article allows comparable and reliable results between and within observers to be obtained. From this and other studies it can be concluded that the method also has a good prognostic value for the prediction of expected IVF fertilization, the hamster test and hemizona assay.read more
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Journal ArticleDOI
Prevalence of hyperinsulinaemia among normozoospermic donors at Medfem Clinic, South Africa.
TL;DR: Embryo quality in an IVF programme may be influenced by male insulin levels, and donors with normal insulin levels produced more good quality blastocysts in theIVF programme than those with abnormal insulin levels.
Morphology and function of human spermatozoa
TL;DR: The final author version and the galley proof are versions of the publication after peer review that features the final layout of the paper including the volume, issue and page numbers.
Journal ArticleDOI
Assessment of spermatozoa in fertile alpaca (Vicugna pacos) males: Study of sperm head morphometry using a nonautomated digital method and sperm morphology based on strict criteria.
D Evangelista-Vargas,Shirley Evangelista-Vargas,Martha Valdivia,A Alexei Santiani,A Alexei Santiani +4 more
TL;DR: It is concluded that sperm head morphometry assessment in fertile alpacas using a nonautomated digital method is feasible, and that defects in sperm heads constitute the main morphological alteration (>50% of the sperm population), based on WHO and strict criteria.
Journal ArticleDOI
In vitro fertilization failure: identification of gamete defects by investigation of sperm-zona pellucida binding capacity of unfertilized oocytes.
TL;DR: The results stress the need to develop a sequential analytic programme for those couples with repeated total or partial fertilization failure, and suggest poor sperm binding potential of spermatozoa may be the cause.