Journal ArticleDOI
The evaluation of morphological characteristics of human spermatozoa according to stricter criteria
TLDR
It can be concluded that the method developed in the laboratory and which resulted in the use of stricter criteria for the evaluation of sperm morphology is a practical, reliable and repeatable method and has a good prognostic value for the prediction of expected IVF fertilization, the hamster test and hemizona assay.Abstract:
The evaluation of the morphology of human spermatozoa varies widely between and sometimes even within laboratories. The purpose of this study was to determine whether the method that has been developed in our laboratory and which resulted in the use of stricter criteria for the evaluation of sperm morphology is a practical, reliable and repeatable method and to establish the within and between observer variations. The criteria used for a 'normal' spermatozoon are based on the appearance of spermatozoa found in the mucus of the upper endocervical canal. The results of the morphological evaluations of 26 samples by four observers were statistically analysed by various methods. The method of Barnett showed a high degree of relative accuracy between observers with error variances of between 2.89 and 19.67 as well as high Spearman rank correlation coefficients of between 0.8675 and 0.6537 (P less than 0.0003). The Spearman correlation coefficient for 15 duplicate evaluations by one observer was 0.9650 (P less than 0.0001) while the coefficients of variation for repeated evaluations of single samples were also within acceptable limits. Based on these results, the method described in this article allows comparable and reliable results between and within observers to be obtained. From this and other studies it can be concluded that the method also has a good prognostic value for the prediction of expected IVF fertilization, the hamster test and hemizona assay.read more
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Journal ArticleDOI
Intake of Fruits and Vegetables with Low-to-Moderate Pesticide Residues Is Positively Associated with Semen-Quality Parameters among Young Healthy Men
Yu-Han Chiu,Audrey J. Gaskins,Paige L. Williams,Jaime Mendiola,Niels Jørgensen,Hagai Levine,Russ Hauser,Shanna H. Swan,Jorge E. Chavarro +8 more
TL;DR: The consumption of fruit and vegetables with low-to-moderate pesticide residues was positively related to sperm counts in young men unselected by fertility status, suggesting that pesticide residues may modify the beneficial effects of Fruit and vegetable intake on semen quality.
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What should it take to describe a substance or product as ‘sperm-safe’
David Mortimer,Christopher L.R. Barratt,Lars Björndahl,Christiaan De Jager,Anne M. Jequier,Charles H. Muller +5 more
TL;DR: It is concluded that products can only be claimed to be 'sperm-safe' after performing objective, properly designed experimental studies; extrapolation from supposed predicate products or other assumptions cannot be trusted.
Journal ArticleDOI
Selection of normal spermatozoa with a vacuole‐free head (x6300) improves selection of spermatozoa with intact DNA in patients with high sperm DNA fragmentation rates
I. Hammoud,Florence Boitrelle,F. Ferfouri,François Vialard,M. Bergere,B. Wainer,Marc Bailly,Martine Albert,Jacqueline Selva +8 more
TL;DR: For patients with high sperm DNA fragmentation rates, selection of normal spermatozoa with a vacuole‐free head (6300×) yields the greatest likelihood of obtaining spermatozoon with non‐fragmented DNA.
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Semen quality and sex hormones among organic and traditional Danish farmers. ASCLEPIOS Study Group.
TL;DR: Despite slight differences in concentrations of reproductive hormones, no significant differences in conventional measures of semen quality were found between organic and traditional farmers.
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Mediterranean and western dietary patterns are related to markers of testicular function among healthy men.
Ana Cutillas-Tolín,Lidia Mínguez-Alarcón,Jaime Mendiola,José J. López-Espín,Niels Jørgensen,Eva María Navarrete-Muñoz,Alberto M. Torres-Cantero,Jorge E. Chavarro +7 more
TL;DR: It is suggested that traditional Mediterranean diets may have a positive impact on male reproductive potential according to the observed differences in semen quality, reproductive hormone levels and testicular volume.