Journal ArticleDOI
The evaluation of morphological characteristics of human spermatozoa according to stricter criteria
TLDR
It can be concluded that the method developed in the laboratory and which resulted in the use of stricter criteria for the evaluation of sperm morphology is a practical, reliable and repeatable method and has a good prognostic value for the prediction of expected IVF fertilization, the hamster test and hemizona assay.Abstract:
The evaluation of the morphology of human spermatozoa varies widely between and sometimes even within laboratories. The purpose of this study was to determine whether the method that has been developed in our laboratory and which resulted in the use of stricter criteria for the evaluation of sperm morphology is a practical, reliable and repeatable method and to establish the within and between observer variations. The criteria used for a 'normal' spermatozoon are based on the appearance of spermatozoa found in the mucus of the upper endocervical canal. The results of the morphological evaluations of 26 samples by four observers were statistically analysed by various methods. The method of Barnett showed a high degree of relative accuracy between observers with error variances of between 2.89 and 19.67 as well as high Spearman rank correlation coefficients of between 0.8675 and 0.6537 (P less than 0.0003). The Spearman correlation coefficient for 15 duplicate evaluations by one observer was 0.9650 (P less than 0.0001) while the coefficients of variation for repeated evaluations of single samples were also within acceptable limits. Based on these results, the method described in this article allows comparable and reliable results between and within observers to be obtained. From this and other studies it can be concluded that the method also has a good prognostic value for the prediction of expected IVF fertilization, the hamster test and hemizona assay.read more
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Journal ArticleDOI
Kruger strict morphology and post-thaw progressive motility in cryopreserved human spermatozoa
TL;DR: Kruger strict morphology assessment was a better predictor of the progressive motility recovery rate following the freezing‐thaw procedure than parameters of conventional semen analysis.
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Morphology of testicular spermatozoa obtained by testicular sperm extraction in obstructive and nonobstructive azoospermic men and its relation to fertilization success in the in vitro fertilization-intracytoplasmic sperm injection system.
Haim Yavetz,Leah Yogev,Sandra E. Kleiman,Amnon Botchan,Ron Hauser,Joseph B. Lessing,Gedalia Paz,Ronni Gamzu +7 more
TL;DR: It can be concluded that a relatively high portion of testicular sperm are morphologically normal, and the higher rate of normal spermatozoa in obstructive azoospermia compared with nonobstructive spermatozosa suggests that the factors leading to azoOSpermia may affecttesticular sperm morphology.
Journal ArticleDOI
4,977‐bp human mitochondrial DNA deletion is associated with asthenozoospermic infertility in Jordan
Mazhar Salim Al Zoubi,Khalid M. Al-Batayneh,Mohammad Alsmadi,Mitri Rashed,Bahaa Al-Trad,Wesam Al Khateeb,Alaa A. A. Aljabali,Osama Otoum,Mohammad M. Al-Talib,Osamah Batiha +9 more
TL;DR: A strong association between 4,977‐bp mtDNA deletion and asthenozoospermia in the Jordanian population was underscored, highlighting the importance of knowing the carrier and removal status of mtDNA in relation to male infertility.
Journal ArticleDOI
Morphometric dimensions of the stallion sperm head depending on the staining method used
TL;DR: The results obtained indicate that in the case of staining with SpermBlue® and with eosin+gentian complex, the morphometry of the sperm head seems to be most similar to that observed in unstained smears.
Journal ArticleDOI
Reassessment of sperm morphology of archival semen smears from the period 1980--1994.
TL;DR: Multiple linear regression analysis of the re-analysed data showed no effect of the year of sample delivery on sperm morphology between the years 1980 and 1994, however, there was a significant decline in the proportion of normal spermatozoa with later year of men's birth.