Journal ArticleDOI
The evaluation of morphological characteristics of human spermatozoa according to stricter criteria
TLDR
It can be concluded that the method developed in the laboratory and which resulted in the use of stricter criteria for the evaluation of sperm morphology is a practical, reliable and repeatable method and has a good prognostic value for the prediction of expected IVF fertilization, the hamster test and hemizona assay.Abstract:
The evaluation of the morphology of human spermatozoa varies widely between and sometimes even within laboratories. The purpose of this study was to determine whether the method that has been developed in our laboratory and which resulted in the use of stricter criteria for the evaluation of sperm morphology is a practical, reliable and repeatable method and to establish the within and between observer variations. The criteria used for a 'normal' spermatozoon are based on the appearance of spermatozoa found in the mucus of the upper endocervical canal. The results of the morphological evaluations of 26 samples by four observers were statistically analysed by various methods. The method of Barnett showed a high degree of relative accuracy between observers with error variances of between 2.89 and 19.67 as well as high Spearman rank correlation coefficients of between 0.8675 and 0.6537 (P less than 0.0003). The Spearman correlation coefficient for 15 duplicate evaluations by one observer was 0.9650 (P less than 0.0001) while the coefficients of variation for repeated evaluations of single samples were also within acceptable limits. Based on these results, the method described in this article allows comparable and reliable results between and within observers to be obtained. From this and other studies it can be concluded that the method also has a good prognostic value for the prediction of expected IVF fertilization, the hamster test and hemizona assay.read more
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MALE INFERTILITY: Evaluation and Nonsurgical Therapy
Alex T. Chuang,Stuart S. Howards +1 more
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Andrology: The use of hemizona assay in the evaluation of the optimal sperm preparation technique
TL;DR: The results support the conclusion that in-vitro sperm preparation methods can affect sperm binding to the zona pellucida and the hemizona assay can be used in selecting the optimal sperm preparation method prior to its use for assisted reproductive techniques.
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Differential expression of mRNA aromatase in ejaculated spermatozoa from infertile men in relation to either asthenozoospermia or teratozoospermia.
TL;DR: The evaluation of the relationship between the expression of aromatase, sperm quality and seminal neutral α–glucosidase marker in semen of Tunisian infertile men highlighted the involvement of arom atase in motility and morphology of spermatozoa.
Dissertation
Infertilité masculine : fragmentation de l'ADN spermatique, ségrégation méiotique et facteurs génétiques
TL;DR: Dans la premiere partie, l'equipement chromosomique et the fragmentation de I'ADN spermatique dans les gametes d'hommes infertiles ont ete etudies par the technique FISH et TUNEL.