Journal ArticleDOI
The evaluation of morphological characteristics of human spermatozoa according to stricter criteria
TLDR
It can be concluded that the method developed in the laboratory and which resulted in the use of stricter criteria for the evaluation of sperm morphology is a practical, reliable and repeatable method and has a good prognostic value for the prediction of expected IVF fertilization, the hamster test and hemizona assay.Abstract:
The evaluation of the morphology of human spermatozoa varies widely between and sometimes even within laboratories. The purpose of this study was to determine whether the method that has been developed in our laboratory and which resulted in the use of stricter criteria for the evaluation of sperm morphology is a practical, reliable and repeatable method and to establish the within and between observer variations. The criteria used for a 'normal' spermatozoon are based on the appearance of spermatozoa found in the mucus of the upper endocervical canal. The results of the morphological evaluations of 26 samples by four observers were statistically analysed by various methods. The method of Barnett showed a high degree of relative accuracy between observers with error variances of between 2.89 and 19.67 as well as high Spearman rank correlation coefficients of between 0.8675 and 0.6537 (P less than 0.0003). The Spearman correlation coefficient for 15 duplicate evaluations by one observer was 0.9650 (P less than 0.0001) while the coefficients of variation for repeated evaluations of single samples were also within acceptable limits. Based on these results, the method described in this article allows comparable and reliable results between and within observers to be obtained. From this and other studies it can be concluded that the method also has a good prognostic value for the prediction of expected IVF fertilization, the hamster test and hemizona assay.read more
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Dissertation
Human subfertility: explorative studies on some pathophysiologic factors in semen and follicular fluid.
TL;DR: It is hypothesized in literature that matrix degradation by the plasminogen activator system and vascularization induced by the vascular endothelial growth factor (VEGF) are causally involved in subfertility.
Book ChapterDOI
Novel markers of male infertility.
Michael Funaro,Darius A. Paduch +1 more
TL;DR: Next-generation genome sequencing and the discovery of small noncoding RNAs in sperm already are changing the field and permit further insight into the biology of male reproduction as well as offer new diagnostic tests.
Journal ArticleDOI
Protocol for developing a core outcome set for male infertility research: an international consensus development study
Michael P. Rimmer,Ruth A Howie,Richard A. Anderson,Christopher L.R. Barratt,Kurt T. Barnhart,Yusuf Beebeejaun,Ricardo Pimenta Bertolla,S. Bhattacharya,Lars Björndahl,Pietro Bortoletto,Robert E. Brannigan,Astrid E. P. Cantineau,Ettore Caroppo,Barbara Collura,Kevin Coward,Michael L. Eisenberg,Christian De Geyter,Dimitrios G. Goulis,Ralf R Henkel,Vu N A Ho,Alayman Hussein,Carin Huyser,Jozef H. Kadijk,Mohan S Kamath,Shadi Khashaba,Yoshitomo Kobori,Julia Kopeika,Tansu Kucuk,Saturnino Luján,Thabo Matsaseng,R. S. Mathur,Kevin McEleny,Rod T. Mitchell,Ben W.J. Mol,Alfred Murage,Ernest Hung Yu Ng,Allan A. Pacey,Antti Perheentupa,Stefan S. du Plessis,Nathalie Rives,I Sarris,Peter N. Schlegel,Majid Shabbir,Maciej Smiechowski,Venkatesh Subramanian,Sesh Kamal Sunkara,Basil C Tarlarzis,Frank Tüttelmann,Andy Vail,Madelon van Wely,Mónica H. Vazquez-Levin,Lan N. Vuong,Alex Y. Wang,Rui Wang,Armand Zini,Cindy Farquhar,Craig Niederberger,James M N Duffy +57 more
TL;DR: An international steering group, including the relevant stakeholders outlined above, has been established to guide the development of this core outcome set, which will establish standardized definitions and recommend high-quality measurement instruments for individual core outcomes.
Book ChapterDOI
Male infertility in chronic urogenital infections and inflammation with special reference to ejaculate findings
Journal ArticleDOI
Drawbacks of the current practice.
TL;DR: The pitfalls of the current practice in using intracytoplasmic sperm injection (ICSI) in bypassing male factors on one hand, while elaborating on the current limitations of SDF testing on the other are discussed.