The Functional Properties of Barrel Cortex Neurons Projecting to the Primary Motor Cortex
Takashi R. Sato,Karel Svoboda +1 more
TLDR
The view that intermingled neurons in primary sensory areas send specific stimulus features to different parts of the brain is supported, including those projecting to primary motor cortex and secondary somatosensory area.Abstract:
Nearby neurons, sharing the same locations within the mouse whisker map, can have dramatically distinct response properties. To understand the significance of this diversity, we studied the relationship between the responses of individual neurons and their projection targets in the mouse barrel cortex. Neurons projecting to primary motor cortex (MI) or secondary somatosensory area (SII) were labeled with red fluorescent protein (RFP) using retrograde viral infection. We used in vivo two-photon Ca(2+) imaging to map the responses of RFP-positive and neighboring L2/3 neurons to whisker deflections. Neurons projecting to MI displayed larger receptive fields compared with other neurons, including those projecting to SII. Our findings support the view that intermingled neurons in primary sensory areas send specific stimulus features to different parts of the brain.read more
Citations
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Optimization of a GCaMP calcium indicator for neural activity imaging.
Jasper Akerboom,Tsai Wen Chen,Trevor J. Wardill,Lin Tian,Lin Tian,Jonathan S. Marvin,Sevinç Mutlu,Sevinç Mutlu,Nicole Carreras Calderón,Nicole Carreras Calderón,Nicole Carreras Calderón,Federico Esposti,Bart G. Borghuis,Bart G. Borghuis,Xiaonan Richard Sun,Andrew Gordus,Michael B. Orger,Michael B. Orger,Ruben Portugues,Florian Engert,John J. Macklin,Alessandro Filosa,Aman Aggarwal,Aman Aggarwal,Rex Kerr,Ryousuke Takagi,Sebastian Kracun,Eiji Shigetomi,Baljit S. Khakh,Herwig Baier,Leon Lagnado,Samuel S.-H. Wang,Cornelia I. Bargmann,Bruce E. Kimmel,Vivek Jayaraman,Karel Svoboda,Douglas S. Kim,Eric R. Schreiter,Eric R. Schreiter,Loren L. Looger +39 more
TL;DR: GCaMP5 fluorescence provides a more reliable measure of neuronal activity than its predecessor GCaMP3, which allows more sensitive detection of neural activity in vivo and may find widespread applications for cellular imaging in general.
Journal ArticleDOI
The neocortical circuit: themes and variations
TL;DR: Similarities in neocortical circuit organization across areas and species suggest a common strategy to process diverse types of information, including sensation from diverse modalities, motor control and higher cognitive processes.
Journal ArticleDOI
Multiple dynamic representations in the motor cortex during sensorimotor learning
Daniel Huber,Diego A. Gutnisky,Simon Peron,Daniel H. O'Connor,J. S. Wiegert,Lin Tian,Thomas G. Oertner,Loren L. Looger,Karel Svoboda +8 more
TL;DR: The results suggest that ensembles of motor cortex neurons couple sensory input to multiple, related motor programs during learning, and that population-level representations of task-related licking strengthened in trained mice.
Journal ArticleDOI
A motor cortex circuit for motor planning and movement
TL;DR: The conversion of preparatory activity to movement within a structured motor cortex circuit is dissected and it is shown that layer 5 neurons projecting within the cortex have unbiased laterality.
Journal ArticleDOI
Long-range neuronal circuits underlying the interaction between sensory and motor cortex.
TL;DR: Channelrhodopsin-2 (ChR2) expression is used, combined with anterograde and retrograde labeling, to map connections between barrel cortex and pyramidal neurons in mouse motor cortex, finding that barrel cortex axons preferentially targeted upper layer neurons in motor cortex; input to neurons projecting back to barrel cortex was particularly strong.
References
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In vivo two-photon calcium imaging of neuronal networks
TL;DR: In vivo two-photon calcium imaging recordings indicated that whisker deflection-evoked Ca2+ transients occur in a subset of layer 2/3 neurons of the barrel cortex, demonstrating the suitability of this technique for real-time analyses of intact neuronal circuits with the resolution of individual cells.
Journal ArticleDOI
ScanImage: Flexible software for operating laser scanning microscopes
Thomas A. Pologruto,Thomas A. Pologruto,Bernardo L. Sabatini,Bernardo L. Sabatini,Karel Svoboda +4 more
TL;DR: This work describes a simple, software-based approach to operating a laser scanning microscope without the need for custom data acquisition hardware and quantifies the effectiveness of the data acquisition and signal conditioning algorithm under a variety of conditions.