Journal ArticleDOI
The human gene for the β subunit of nerve growth factor is located on the proximal short arm of chromosome 1
TLDR
Fragments of the recently cloned human gene for the beta subunit of nerve growth factor (beta-NGF) were used as hybridization probes in analyzing two sets of rodent-human somatic cell hybrids for the presence of human beta- NGF sequences.Abstract:
Fragments of the recently cloned human gene for the beta subunit of nerve growth factor (beta-NGF) were used as hybridization probes in analyzing two sets of rodent-human somatic cell hybrids for the presence of human beta-NGF sequences. Results from the first set of hybrids assigned the human beta-NGF gene to chromosome 1 and ruled out the presence of sequences of comparable homology on any other chromosome. With the second set of hybrids, which contained seven different, but overlapping, regions of chromosome 1, the NGF locus was mapped to band 1p22.read more
Citations
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The nerve growth factor 35 years later
TL;DR: The field of experimental embryology, which had been enthusiastically acclaimed in the mid-thirties, suffered from a sharp decrease in the enthusiasm that had inflamed the pioneers in this field, ever since R. G. Harrison delivered his celebrated lecture at the Royal Society in London in 1935.
Journal ArticleDOI
The nerve growth factor: thirty-five years later.
TL;DR: A brief account of the state-of-the-art of experimental neuroembryology in the forties, when interest in this approach to the study of the developing nervous system was waning, is a prerequisite for understanding the sudden unforeseeable turn of events which resulted in the discovery of the Nerve Growth Factor.
Journal ArticleDOI
The role of neuronal growth factors in neurodegenerative disorders of the human brain
Bronwen Connor,Mike Dragunow +1 more
TL;DR: The role members of the nerve growth factor family (NGF, BDNF and NT-3) and trk receptors as well as additional growth factors (GDNF, TGF-alpha and IGF-I) may play in neurodegenerative disorders of the human brain are examined.
Journal ArticleDOI
Nerve growth factor and Alzheimer's disease.
Franz Hefti,William J. Weiner +1 more
TL;DR: Findings in experimental animals indicate that nerve growth factor (NGF), a well‐characterized protein, acts as a neurotrophic factor for cholinergic neurons of the basal forebrain and suggest that increasing the availability of NGF to human cholineric cells might promote their survival in certain disease processes.
Journal ArticleDOI
Human chromosomal mapping of genes for insulin-like growth factors I and II and epidermal growth factor.
TL;DR: The chromosomal mapping of the human genes for IGF-I and IGF-II is reported, and it is speculated that these map assignments reflect the existence of gene families involved in growth control.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
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Platelet-derived growth factor is structurally related to the putative transforming protein p28sis of simian sarcoma virus.
Michael D. Waterfield,Geoffrey T. Scrace,Nigel Whittle,Paul Stroobant,Ann Johnsson,Åke Wasteson,Bengt Westermark,Carl-Henrik Heldin,Jung San Huang,Thomas F. Deuel +9 more
TL;DR: A partial amino acid sequence of human platelet-derived growth factor, the major mitogen in serum for cells of mesenchymal origin, shows virtual identity with the predicted sequence of p28sis, the putative transforming protein of simian sarcoma virus (SSV).
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Simian sarcoma virus onc gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor
Russell F. Doolittle,Michael W. Hunkapiller,Leroy Hood,Sushilkumar G. Devare,Keith C. Robbins,Stuart A. Aaronson,Harry N. Antoniades +6 more
TL;DR: The demonstrating of extensive sequence similarity between the transforming protein derived from the simian sarcoma virus onc gene, v-sis, and a human platelet-derived growth factor shows that this protein could be a factor active transiently during normal cell growth.
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Changes in size and secondary structure of the ribosomal transcription unit during vertebrate evolution
TL;DR: Analysis of the double-stranded, S 1 -resistant fragments indicates that there is a direct relationship between the hairpin loops seen in the electron microscope and secondary structure in aqueous solution.
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Human β -nerve growth factor gene sequence highly homologous to that of mouse
TL;DR: The isolation of mouse β-NGF complementary DNA (cDNA) is described and its nucleotide sequence is presented, which predicts a prepro-β- NGF molecule of Mr 27,000 (27K) and a pro-β -NGF molecules of Mr 25K.