Journal ArticleDOI
The inhibition of pyruvate kinase by ATP: a Mg++ buffer system for use in enzyme studies.
P.D. Boyer,P.D. Boyer +1 more
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A simple procedure for “buffering” the free Mg++ concentration in an enzyme system is described, based on use of moderately dissociated Mg+ salts, which demonstrated to be largely competitive with phosphoenolpyruvate and independent of removal of free M g++ by the ATP.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1969-03-10. It has received 29 citations till now. The article focuses on the topics: Pyruvate kinase & Pyruvate dehydrogenase kinase.read more
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Book ChapterDOI
Concentrations of Metabolites and Binding Sites. Implications in Metabolic Regulation
Alberto Sols,R. Marco +1 more
TL;DR: This chapter discusses the implications in metabolic regulation because of the concentrations of metabolites and binding sites in the eukaryotic cell of higher organisms, in which practically all important metabolic reactions occur.
Journal ArticleDOI
Regulation of Liver Pyruvate Kinase and the Phosphoenolpyruvate Crossroads
TL;DR: The hypothesis that an isoenzyme of pyruvate kinase present in liver and kidney has evolved with built-in specific regulatory mechanisms in order to prevent the diversion of phosphoenolpyruvates from its way to glucose in glucoenogenic situations is strongly supported.
Journal ArticleDOI
Transcription In Vitro by Reovirus-Associated Ribonucleic Acid-Dependent Polymerase
TL;DR: Kinetic and pulse-chase studies indicate that the different-sized products are synthesized simultaneously but at rates which are in the order: small > medium > large.
Journal ArticleDOI
Some effects of ionophores for divalent cations on blood platelets. Comparison with the effects of thrombin.
P. Massini,Ernst F. Lüscher +1 more
TL;DR: Results suggest that an increase of the concentration of Ca2+ in the cytoplasm plays a role in the activation of blood platelets.
References
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Journal ArticleDOI
The relationships between substrates and enzymes of glycolysis in brain.
TL;DR: The combined analytical and kinetic information makes it seem likely that along the glycol.ytic pathway in mouse brain (a) no step is limited by the amount of enzyme present; (b) equilibrium is approximated at five or possibly six steps even during maximal glycolysis; (c) one step never reaches equilibrium; and (d) the hexokinase and phosphofructokinase steps are the only points at which there is absolute control of Glycolytic flux.
Journal ArticleDOI
Crystallization, Characterization and Metabolic Regulation of Two Types of Pyruvate Kinase Isolated from Rat Tissues
Journal ArticleDOI
The correlation of reaction kinetics and substrate binding with the mechanism of pyruvate kinase.
TL;DR: Kinetic and binding studies with pyruvate kinase should reveal if substrate interactions occur in a manner consistent with a direct transfer mechanism, and it is the principal purpose of this paper to report the results of such studies.
Journal ArticleDOI
The inhibition of pyruvate kinase by ATP.
TL;DR: It was shown that the inhibition of pyruvate kinase by ATP was abolished completely when sufficient magnesium was added and the inhibition reported by previous workers was attributable to the magnesium complexing properties of ATP.
Journal ArticleDOI
Die Erdalkalikomplexe von Adenosintetraphosphat, Glycerophosphat und Fructosephosphat†
G. Schwarzenbach,G. Anderegg +1 more
TL;DR: In this article, the stability constants of the complexes with glycerinphosphate, fructose phosphate and adenosintetraphosphate have been determined, and the stability of these complexes with adenosin-traphoric acid has been shown to be stable.