Journal ArticleDOI
The locus of sequence-directed and protein-induced DNA bending
Hen-Ming Wu,Donald M. Crothers +1 more
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The bending locus of trypanosome kinetoplast DNA, identified by gel electrophoresis, has tracts of a simple repeat sequence symmetrically distributed about it, with a repeat interval of 10 base pairs.Abstract:
The bending locus of trypanosome kinetoplast DNA, identified by gel electrophoresis, has tracts of a simple repeat sequence (CA5–6 T) symmetrically distributed about it, with a repeat interval of 10 base pairs The analogous bending induced when catabolite gene activating protein binds to its recognition sequence near the promoter of the Escherichia coli lac operon is centred on a site about 5–7 base pairs away from the centre of the protein binding siteread more
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Journal ArticleDOI
An Escherichia coli recBCsbcBrecF host permits the deletion-resistant propagation of plasmid clones containing the 5'-terminal palindrome of minute virus of mice.
Robert Boissy,Caroline R. Astell +1 more
TL;DR: Characterization of plasmid DNA isolated from a recBCsbcBrecF host indicated deletion-resistant propagation of the intact species, and sequence analysis of unamplified and chloramphenicol (Cm)-amplify plasmids obtained from these clones confirmed the integrity of the palindromic region of the viral DNA insert.
Journal ArticleDOI
Conformational changes in a replication origin induced by an initiator protein
TL;DR: The replication initiator protein of the plasmid R6K binds to seven contiguous 22 bp direct repeats that form an indispensable part of the three replication origins alpha, beta, and gamma.
Journal ArticleDOI
POU proteins bend DNA via the POU-specific domain
TL;DR: In a circular permutation assay, several POU proteins, including Oct‐1, Oct‐2A,Oct‐6 and Pit‐ 1, demonstrated a position dependent mobility of the protein‐DNA complexes, suggesting induction of DNA bending.
Journal ArticleDOI
Dimerization of Escherichia coli UvrA and its binding to undamaged and ultraviolet light damaged DNA.
TL;DR: The kinetics of association and dissociation reactions indicate that the primary effects of ATP are on the extent of UvrA dimerization rather than on the properties of the Uvr a-uvDNA complex.
Book ChapterDOI
Using hydroxyl radical to probe DNA structure.
Mary Ann Price,Thomas D. Tullius +1 more
TL;DR: This chapter describes the application of the hydroxyl radical as a reagent for elucidating the structural details of DNA by abstracting a hydrogen atom from a deoxyribose residue in the DNA backbone.
References
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Journal ArticleDOI
A rapid boiling method for the preparation of bacterial plasmids
David S. Holmes,Michael Quigley +1 more
TL;DR: A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed and can be readily adapted for the preparation of plasmid from liter cultures with quantitative yields.
Journal ArticleDOI
Structure of catabolite gene activator protein at 2.9 Å resolution suggests binding to left-handed B-DNA
David B. McKay,Thomas A. Steitz +1 more
TL;DR: The 2.9 Å resolution crystal structure of Escherichia coli catabolite gene activator protein (CAP) completed with cyclic AMP reveals two distinct structural domains separated by a cleft, suggesting that the CAP conversion of right- to left-handed DNA in a closed supercoil, is what activates transcription by RNA polymerase.
Journal ArticleDOI
Bent helical structure in kinetoplast DNA.
TL;DR: It is proposed that this molecule contains a region of systematically bent B-DNA, which accounts for the fragment's difficulty in snaking through the pores of a polyacrylamide gel, its ease in diffusing into Sephacryl beads, and its smaller rotational relaxation time.
Journal ArticleDOI
Genetic regulation: the Lac control region.
TL;DR: Identification of mutant variants of the sequence combined with the in vitro biochemical studies of others has allowed us to tentatively identify the recognition site for each of these proteins, and to suggest how CAP might act at a distance to affect the interaction of RNA polymerase with the promoter.
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