Journal ArticleDOI
The locus of sequence-directed and protein-induced DNA bending
Hen-Ming Wu,Donald M. Crothers +1 more
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The bending locus of trypanosome kinetoplast DNA, identified by gel electrophoresis, has tracts of a simple repeat sequence symmetrically distributed about it, with a repeat interval of 10 base pairs.Abstract:
The bending locus of trypanosome kinetoplast DNA, identified by gel electrophoresis, has tracts of a simple repeat sequence (CA5–6 T) symmetrically distributed about it, with a repeat interval of 10 base pairs The analogous bending induced when catabolite gene activating protein binds to its recognition sequence near the promoter of the Escherichia coli lac operon is centred on a site about 5–7 base pairs away from the centre of the protein binding siteread more
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Role of the DNA/membrane complex in prokaryotic DNA replication.
TL;DR: Les complexes DNA-membrane: article de synthese sur les analyses biochimiques, genetiques and ultrastructurales concernant les bacteries, les plasmides ainsi que les bacteriophages.
Journal ArticleDOI
Refinement of the solution structure of the ribonucleotide 5'r(GCAUGC)2: combined use of nuclear magnetic resonance and restrained molecular dynamics.
TL;DR: In this article, the solution structure of the self-complementary hexamer 5'r (GCAUGC)2 is investigated by means of nuclear magnetic resonance spectroscopy and restrained molecular dynamics.
Journal ArticleDOI
Hoechst 33258, distamycin A, and high mobility group protein I (HMG-I) compete for binding to mouse satellite DNA.
TL;DR: Gel mobility shift assays show that HMG-I forms specific complexes with satellite DNA and that the formation of these complexes is competed for by both Hoechst and distamycin.
Journal Article
Interconvertible Lac Repressor--DNA Loops Revealed by Single-Molecule Experiments
TL;DR: In vitro loop formation mediated by Escherichia coli lactose repressor is examined using single-molecule structural and kinetics methods, and repeated spontaneous transitions between two distinct loop structures were observed in individual protein–DNA complexes.
Journal ArticleDOI
DNA bending by the bulge defect.
Janet A. Rice,Donald M. Crothers +1 more
TL;DR: Comparative gel electrophoresis measurements were used to characterize DNA bending in molecules containing an extra adenosine on one strand, the so-called bulge defect, and concluded that the bulge bend is 21 degrees +/- 3 degrees, primarily in the direction of tilt away from the bulged base.
References
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Journal ArticleDOI
A rapid boiling method for the preparation of bacterial plasmids
David S. Holmes,Michael Quigley +1 more
TL;DR: A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed and can be readily adapted for the preparation of plasmid from liter cultures with quantitative yields.
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Structure of catabolite gene activator protein at 2.9 Å resolution suggests binding to left-handed B-DNA
David B. McKay,Thomas A. Steitz +1 more
TL;DR: The 2.9 Å resolution crystal structure of Escherichia coli catabolite gene activator protein (CAP) completed with cyclic AMP reveals two distinct structural domains separated by a cleft, suggesting that the CAP conversion of right- to left-handed DNA in a closed supercoil, is what activates transcription by RNA polymerase.
Journal ArticleDOI
Bent helical structure in kinetoplast DNA.
TL;DR: It is proposed that this molecule contains a region of systematically bent B-DNA, which accounts for the fragment's difficulty in snaking through the pores of a polyacrylamide gel, its ease in diffusing into Sephacryl beads, and its smaller rotational relaxation time.
Journal ArticleDOI
Genetic regulation: the Lac control region.
TL;DR: Identification of mutant variants of the sequence combined with the in vitro biochemical studies of others has allowed us to tentatively identify the recognition site for each of these proteins, and to suggest how CAP might act at a distance to affect the interaction of RNA polymerase with the promoter.
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Empirical estimation of protein-induced DNA bending angles: applications to λ site-specific recombination complexes
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