Journal ArticleDOI
The locus of sequence-directed and protein-induced DNA bending
Hen-Ming Wu,Donald M. Crothers +1 more
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The bending locus of trypanosome kinetoplast DNA, identified by gel electrophoresis, has tracts of a simple repeat sequence symmetrically distributed about it, with a repeat interval of 10 base pairs.Abstract:
The bending locus of trypanosome kinetoplast DNA, identified by gel electrophoresis, has tracts of a simple repeat sequence (CA5–6 T) symmetrically distributed about it, with a repeat interval of 10 base pairs The analogous bending induced when catabolite gene activating protein binds to its recognition sequence near the promoter of the Escherichia coli lac operon is centred on a site about 5–7 base pairs away from the centre of the protein binding siteread more
Citations
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Journal ArticleDOI
Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees.
TL;DR: The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 90 degrees.
Journal ArticleDOI
Co-crystal structure of TBP recognizing the minor groove of a TATA element
TL;DR: The three-dimensional structure of a TATA-box binding polypeptide complexed with the TATA element of the adenovirus major late promoter has been determined by X-ray crystallography at 2.25 Å resolution.
Journal ArticleDOI
DNA bending at adenine . thymine tracts.
TL;DR: Intrinsic bending of DNA molecules results from local structural polymorphism in regions of homopolymeric dA · dT which are at least 4 base pairs long; the A · T tracts must be repeated in phase with the helix screw.
Book
DNA Structure and Function
TL;DR: This chapter discusses the structure and function of DNA, which is a right handed helix of two individual antiparallel DNA strands that is the source of all intrinsic genetic information.
Journal ArticleDOI
Sequence periodicities in chicken nucleosome core DNA.
TL;DR: The rotational positioning of DNA about the histone octamer appears to be determined by certain sequence-dependent modulations of DNA structure, and it is observed that long runs of homopolymer (dA) X (dT) prefer to occupy the ends of core DNA, five to six turns away from the dyad.
References
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Journal ArticleDOI
Polynucleotide block polymers consisting of a DNA.RNA hybrid joined to a DNA.DNA duplex. Synthesis and characterization of dGn.rCidCk duplexes.
Erik Selsing,Robert D. Wells +1 more
TL;DR: The NMR studies and the results described herein indicate that these block polymers are linear duplexes with two adjoining conformations yet are hydrogen-bonded and base-stacked throughout with minimal disruption of the helix at the junction of the two conformations.
Journal ArticleDOI
Selective effects of MgCl2 and temperature on the initiation of transcription at lac, gal, and lambda promoters.
TL;DR: The studies suggest that the DNA of the lac and gal promoter regions is more resistant to denaturation than theDNA of phage promoter regions, and Cyclic AMP and CRP act to decrease this stability, stimulating open complex formation under conditions unfavorable for DNA melting.
Journal ArticleDOI
Specific DNA binding of the cAMP receptor protein within the lac operon stabilizes double-stranded DNA in the presence of cAMP.
TL;DR: The cAMP‐CRP complex exerts a specific effect with a region of approximately 36 bp, comprising the specific CRP binding site and a neighbouring region of DNA, being stabilized, resulting in a progressive stabilization of the double‐stranded DNA.
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Empirical estimation of protein-induced DNA bending angles: applications to λ site-specific recombination complexes
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