Journal ArticleDOI
The locus of sequence-directed and protein-induced DNA bending
Hen-Ming Wu,Donald M. Crothers +1 more
Reads0
Chats0
TLDR
The bending locus of trypanosome kinetoplast DNA, identified by gel electrophoresis, has tracts of a simple repeat sequence symmetrically distributed about it, with a repeat interval of 10 base pairs.Abstract:
The bending locus of trypanosome kinetoplast DNA, identified by gel electrophoresis, has tracts of a simple repeat sequence (CA5–6 T) symmetrically distributed about it, with a repeat interval of 10 base pairs The analogous bending induced when catabolite gene activating protein binds to its recognition sequence near the promoter of the Escherichia coli lac operon is centred on a site about 5–7 base pairs away from the centre of the protein binding siteread more
Citations
More filters
Journal ArticleDOI
Conformational change in the DNA associated with an unusual promoter mutation in a tRNA operon of Salmonella.
Lionello Bossi,Diane M. Smith +1 more
TL;DR: The results indicate the existence of an unusual DNA conformation, probably involving DNA bending, in the region preceding the tRNA operon promoter, which is apparently disrupted by the 3 bp deletion at position -70.
Journal ArticleDOI
Functional domains within the a sequence involved in the cleavage-packaging of herpes simplex virus DNA.
L P Deiss,J Chou,Niza Frenkel +2 more
TL;DR: Comparison of published sequences of concatemeric junctions of several herpesviruses supported a uniform mechanism for the cleavage-packaging process, involving the measurement from two highly conserved blocks of sequences which were homologous to the required Uc and Ub sequences.
Journal ArticleDOI
High-mobility-group 1 protein mediates DNA bending as determined by ring closures
TL;DR: Findings indicate that the role of HMG1 could involve both structure-specific recognition of prebent DNA and distortion of the DNA helix by bending and that the HMG-box domain may actually be responsible for this activity.
Journal ArticleDOI
Allosteric underwinding of DNA is a critical step in positive control of transcription by Hg-MerR.
TL;DR: The magnitude and the direction of the Hg–MerR-induced change in twist angle are consistent with a positive control mechanism involving reorientation of conserved, but suboptimally phased, promoter elements and are inconsistent with a role for torsional stress in formation of an open complex.
Journal ArticleDOI
The unique structure of A-tracts and intrinsic DNA bending.
Tali E. Haran,Udayan Mohanty +1 more
TL;DR: It is shown that very often it is the unique structure of A-tracts that is at the basis of their widespread occurrence in regulatory regions of many organisms and may often be residing in their distinct structure rather than in the global curvature that they induce on sequences containing them.
References
More filters
Journal ArticleDOI
A rapid boiling method for the preparation of bacterial plasmids
David S. Holmes,Michael Quigley +1 more
TL;DR: A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed and can be readily adapted for the preparation of plasmid from liter cultures with quantitative yields.
Journal ArticleDOI
Structure of catabolite gene activator protein at 2.9 Å resolution suggests binding to left-handed B-DNA
David B. McKay,Thomas A. Steitz +1 more
TL;DR: The 2.9 Å resolution crystal structure of Escherichia coli catabolite gene activator protein (CAP) completed with cyclic AMP reveals two distinct structural domains separated by a cleft, suggesting that the CAP conversion of right- to left-handed DNA in a closed supercoil, is what activates transcription by RNA polymerase.
Journal ArticleDOI
Bent helical structure in kinetoplast DNA.
TL;DR: It is proposed that this molecule contains a region of systematically bent B-DNA, which accounts for the fragment's difficulty in snaking through the pores of a polyacrylamide gel, its ease in diffusing into Sephacryl beads, and its smaller rotational relaxation time.
Journal ArticleDOI
Genetic regulation: the Lac control region.
TL;DR: Identification of mutant variants of the sequence combined with the in vitro biochemical studies of others has allowed us to tentatively identify the recognition site for each of these proteins, and to suggest how CAP might act at a distance to affect the interaction of RNA polymerase with the promoter.
Related Papers (5)
Empirical estimation of protein-induced DNA bending angles: applications to λ site-specific recombination complexes
John F. Thompson,Arthur Landy +1 more