The origin of spontaneous mutation in saccharomyces cerevisiae
TLDR
An additive effect on the reduction in spontaneous mutation leads to the conclusion that at least 90% of spontaneous mutations seen in the wild type are caused by mutagenic repair of spontaneous lesions.Abstract:
Characterization of two antimutator loci in yeast shows that both are members of the same mutagenic repair system known to be responsible for almost all induced mutation (LAWRENCE and CHRISTENSEN 1976, 1979a,b; PRAKASH 1976). One of the these newly isolated antimutator mutations is an allele of rev3 (LEMONTT 1971b). Two other alleles of rev3 were tested and were also found to be antimutators. Double mutants carrying rev3 and mutator mutations of rad3, rad51 or rad18 are like rev3 single mutants with respect to spontaneous mutation rate, supporting the hypothesis (HASTINGS, QUAH and VON BORSTEL, 1976) that many mutators in yeast act by channelling spontaneous lesions from accurate to mutagenic repair. However, the enhanced mutation rate seen in a radiation-resistant mutator mutant mut1 is not dependent on REV3, but is dependent on another gene designated ANT1. An additive effect on the reduction in spontaneous mutation, seen in the ant1 rev3 double-mutant strain, leads to the conclusion that at least 90% of spontaneous mutations seen in the wild type are caused by mutagenic repair of spontaneous lesions.read more
Citations
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References
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Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae
Louise Prakash,Satya Prakash +1 more
TL;DR: In isolated mutants sensitive to methyl methanesulfonate (MMS) in Saccharomyces cerevisiae, the cross-sensitivities of these mms mutants to UV and X rays are discussed in terms of their possible involvement in DNA repair.
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Genetical mutants induced by ethyl methanesulfonate in saccharomyces
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Mutants of Yeast Sensitive to Ultraviolet Light
TL;DR: Six uvr mutants of Saccharomyces cerevisiae with hypersensitivity to ultraviolet (UV) light were isolated after mutagen treatment with ethylmethanesulfonate and implementation tests and tetrad analysis indicate that each strain represents mutation in a different gene.
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Effect of Genes Controlling Radiation Sensitivity on Chemically Induced Mutations in SACCHAROMYCES CEREVISIAE.
TL;DR: The effect of 16 different genes (rad) conferring radiation sensitivity on chemically induced reversion in the yeast Saccharomyces cerevisiae was determined in this paper, where the site of reversion used was a well-defined chain initiation mutant mapping in the structural gene coding for iso-1-cytochrome c.
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Ultraviolet-induced reversion of cyc1 alleles in radiation-sensitive strains of yeast: I. rev1 mutant strains☆
TL;DR: The evidence of this kind of allele-specific control of u.v. -induced reversion must be accounted for by any proposed model of the mutagenic process and must also be accommodated by any scheme to test environmental mutagens.