Journal ArticleDOI
The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation
A. Fleck,Hamish N. Munro +1 more
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The use of ultraviolet absorption measurements for the estimation of RNA by the Schmidt-Thannhauser procedure has been examined on samples of rat liver, finding that a 1-h period of digestion in alkali at 37° releases negligible quantities of protein, provided that the concentration of alkali is low.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1962-05-14. It has received 943 citations till now.read more
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Nuclei from Rat Liver: Isolation Method That Combines Purity with High Yield
Günter Blobel,Van R. Potter +1 more
TL;DR: A procedure is described that gives almost quantitative separation of clean nuclei from a homogenate of rat liver and permits further quantitative fractionation of cytoplasmic components.
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Endomembrane trafficking of ras: the CAAX motif targets proteins to the ER and Golgi.
Edwin Choy,Vi K. Chiu,Joseph Silletti,Marianna Feoktistov,Takashi Morimoto,David Michaelson,I E Ivanov,Mark R. Philips +7 more
TL;DR: It is shown that Nras is transiently localized in the Golgi prior to the plasma membrane (PM), and green fluorescent protein (GFP)-tagged Nras illuminated motile, peri-Golgi vesicles, and prolonged BFA treatment blocked PM expression.
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Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum.
Peter Walter,Günter Blobel +1 more
TL;DR: In addition to its previously characterized, six different polypeptide components, signal recognition protein—which functions in protein translocation across and integration into the endoplasmic reticulum membrane—contains a 7S RNA molecule that is required for both structural and functional properties of signal recognition particle.
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Phosphorylation of a 22,000-dalton component of the cardiac sarcoplasmic reticulum by adenosine 3':5'-monophosphate-dependent protein kinase.
TL;DR: Cardiac microsomes were incubated with [gamma-32P]ATP and a cardiac adenosine 3':5'-monophosphate (cyclic AMP)-dependent protein kinase in the presence of ethylene glycol bis(bets-aminoethyl ether)-N,N'-tetraacetic acid to indicate that this protein is associated with the membranes of sarcoplasmic reticulum rather than being a contaminant from other soluble proteins.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues.
TL;DR: The methods used at present for the determination of the higher nucleic acids in organs are based on certain color reactions of their carbohydrate components, but if the quantitative estimations of desoxyribonucleic acid and of ribon nucleic acid could be based on phosphorus determinations rather than on color tests of their carbohydrates components, these difficulties might be avoided.
Journal ArticleDOI
Phosphorus compounds in animal tissues; extraction and estimation of desoxypentose nucleic acid and of pentose nucleic acid.
Journal ArticleDOI
Use of glucose oxidase, peroxidase, and o-dianisidine in determination of blood and urinary glucose
A. St. G. Huggett,Nixon Da +1 more